摘要
转醛醇酶作为磷酸戊糖途径非氧化阶段的关键酶,在调节植物PPP对环境胁迫的应答中起重要作用。该研究应用RACE方法克隆了龙眼(Dimocarpus longan Lour.)花芽中转醛醇酶的基因,获得一段长度为1655bp的cDNA,其中包括一个1320bp的开放阅读框,已登陆GenBank,登陆号为FJ472991(GI:217795375)。将转醛醇酶全长cDNA在大肠杆菌中表达,获得一个约55kD带有组氨酸标签的外源蛋白。RT-PCR结果显示,TALmRNA在龙眼成花逆转花芽中有较明显的表达量上调,说明TAL在一定程度上影响了龙眼正常成花。
The pentose-phosphate pathway （PPP） plays a pivotal role in carbohydrate metabolism, as a key enzyme of the nonoxidative PPP branch, transaldolase showed advertent response to environmental stress regulation. We have isolated a cDNA encoding transaldolase, an enzyme of the pentose-phosphate pathway, from longan （Dimocarpus longan Lour. ）. RACE method was used and a full length of 1 655 bp cDNA, with a 1 320 bp open reading frame, was obtained （GenBank access number： FJ479618）. Transaldolase cDNA was expressed in E. coli, and a 55 kD heterologous protein with histidine tag was obtained. The RT-PCR result showed TAL mRNA had a remarkable up-regulation, indicate the expression of transaldolase influence longan flowering transition in a certain extent.
出处
《北方园艺》
CAS
北大核心
2009年第12期7-10,共4页
Northern Horticulture
基金
国家自然科学基金资助项目(30571293)
福建省自然科学基金资助项目(2007J0045)
教育部博士点基金资助(200803890009)
关键词
龙眼
转醛醇酶
基因
克隆
原核表达
Longan
Transaldolase
Gene
Clone
Prokaryotic expression
