摘要
[目的]研究苦丁茶不同粗提物的抑菌效果。[方法]采用试管二倍稀释法和纸片扩散法,对金黄色葡萄球菌与大肠埃希菌进行体外抑菌试验,并测定其最小抑菌浓度(MIC)、最小杀菌浓度(MBC)和抑菌环直径。[结果]粗提物1抑菌效果较强,高于粗提物2和粗提物3,对金黄色葡萄球菌与大肠埃希菌的MIC分别为3.91和31.25mg/ml。[结论]苦丁茶的不同粗提物均有不同程度的抑菌作用,为苦丁茶开发成新型、安全的抑菌剂提供参考。
[ Objective ] The aim was to study bacteriostatic activity of the different extracts from flex kudingcha C. J. Tseng. [ Method ] By using test tube 2-fold dilution method and Kirby-Baueer Disc Diffusionto, the study conducted inhibitory test on S. aureus and E. coil and determined the minimal inhibitory concentration （MIC） and minimum bactericidal concentration （ MBC ） and the diameter of inhibition zone. [ Result ] The extract 1 had fairly stronger in vitro bacteriostasis activities and than the extract 2 and extract 3. the MIC and MBC was 3.91 and 31.25 mg/ml on S. aureus and E. coll. [ Conclusion] The result showed that the different extracts from liex kudingcha C. J. Tseng had different bacteriostatic activities as different degree,it provides reference for developing new and safe bacteriostatic reagent.
出处
《安徽农业科学》
CAS
北大核心
2009年第34期16838-16840,共3页
Journal of Anhui Agricultural Sciences
基金
广西自然科学基金攻关项目(桂科攻0719004-3H)
关键词
苦丁茶
提取物
体外抑菌作用
liex kudingcha C. J. Tseng
The extract
Bacteriostasis in vitro
