摘要
目的:探讨生长激素(GH)联合氟尿嘧啶(5-Fu)在体外对结肠癌LOVO细胞的影响。方法:将培养的结肠癌LOVO细胞分为对照组、GH组、5-Fu组和GH+5-Fu组,GH组又按剂量分为两个亚组,即GH1终浓度为50ng/mL和GH2终浓度为100 ng/mL;GH+5-Fu组又按剂量分为两个亚组,即GH1+5-Fu(GH 50 ng/mL+5-Fu 100μg/mL)和GH2+5-Fu(GH 100 ng/mL+5-Fu 100μg/mL),加入不同浓度的处理液后24、48和72 h,分别行四基偶氮唑蓝(MTT)比色观察,计算细胞存活率。并于加入不同浓度的处理液24 h后,以流式细胞仪测定细胞周期和凋亡情况。结果:5-Fu组和GH+5-Fu组与对照组比较,细胞存活率明显下降(P<0.05)。GH+5-Fu组与单用5-Fu组比较,细胞生存率均有所降低,但各时间段无显著性差异(P>0.05)。GH组与对照组比较,S期细胞百分比和增殖指数(PI)均增加(P<0.05),G0/G1细胞百分比有所减少(P<0.05)。GH+5-Fu组的S期细胞百分比和PI均降低(P<0.05)。5-Fu组和GH+5-Fu组的细胞凋亡率均升高(P<0.05);GH+5-Fu组与5-Fu组比较,S期细胞百分比、PI和凋亡率均无显著性差异(P>0.05)。结论:rhGH体外作用于结肠癌LOVO细胞,未刺激细胞生长,GH与化疗联合用是安全的。
Objective： To investigate the effects of recombinant human growth hormone （rhGH） and 5- fluorouracil（5-Fu） on human colon carcinoma LOVO cells in vitro. Methods： The LOVO cells during exponential growth stage were harvested and divided into control group, GH group, 5-Fu group and GH ＋ 5-Fu group. According to the dose of GH, the GH group was separated into two sub-groups （50 ng/mL and 100 ng/mL ） and the GH ＋5-Fu group was separated into two sub-groups. With different concentrations of rhGH and/or 5-Fu , the cell survive rates were analyzed by MTT assay after 24 h , 48 h and 72 h and cell cycle and proliferation index （PI） were analyzed by flow cytometry after 24 h. Results： Compared with the control group, the survive rates in 5-Fu and GH ＋ 5-Fu groups were decreased significantly（P 〈0.05）. The significant effects of rhGH on cell cycle kinetics were found in the cell line, Compared with the control group, percentage of S phase and proliferation index （PI） significantly increased（P 〈 0.05 ） and percentage of G0/G1 phase decreased （P 〈 0.05 ） in GH groups. Percentages of cells of S phase and PI significantly decreased in GH ＋ 5-Fu groups （ P 〈 0. 05 ）. Rate of apoptosis increased in 5-Fu and GH ＋ 5-Fu groups （P 〈 0. 05 ）. Compared with the 5-Fu group, there were no statistically significant differences in percentages of cells of S phase and PI and rate of apoptosis between two GH ＋ 5-Fu groups （ P 〉 0.05 ）. Conclusion ： rhGH does not stimulate the LOVO cells proliferation in vitro, and its use is safe when combined with 5-fluorouracil.
出处
《肠外与肠内营养》
CAS
北大核心
2009年第6期343-345,350,共4页
Parenteral & Enteral Nutrition
基金
海南省自然科学基金资助项目(806112)
