摘要
用基质辅助红外激光解吸离子化／傅里叶变换离子回旋共振质谱法（ＩＲＭＡＬＤＩ／ＦＴＩＣＲＭＳ）分析了促黄体素释放激素类似物、血管紧张肽原酶底物及血管紧张肽。分别以２，５二羟基苯甲酸、丁二酸为基质，测定其分子量，测定值与理论计算值的相对误差小于４×１０－６，适当增加激光功率密度，获得了一系列特征碎片离子并进行了归属，从而确证了３种寡肽的一级结构。提示本研究为测定寡肽的分子量和一级结构提供了一个简便、快速。
Luteinizing releasing hormone analog (LRH A), renin substrate tetradecapeptide and angiotensin I were analyzed with infrared matrix assisted laser desorption ionization/Fourier transform ion cyclotron resonance mass spectrometry (IR MALDI/FTICR MS). 2,5 Dihydroxybenzoic (DHB) and succinic acid (SA) were used as matrices. The m/z of oligopeptides were determined with an error less than 4×10 -6 . A series of characteristic fragment ions of peptides were obtained by increasing the laser power density appropriately. Their elemental compositions and the first order structures were confirmed. A sensitive, rapid and accurate method was established for the determination of oligopeptide.
出处
《药学学报》
CAS
CSCD
北大核心
1998年第11期843-848,共6页
Acta Pharmaceutica Sinica
基金
江苏省自然科学基金
关键词
基质辅助
红外激光解吸
质谱法
寡肽
Matrix assisted laser desorption ionization mass spectrometry
Fourier transform/ion cyclotron resonance mass spectrometry
Oligopeptide
Luteinizing releasing hormone analog (LRH A)
Renin substrate tetradecapeptide
Angiotensin I
