摘要
目的观察诱导分化为心肌样细胞的人骨髓间充质干细胞(hMSCs)是否可产生动作电位。方法体外分离培养hM-SCs,5-氮胞苷诱导向心肌细胞(CMs)分化。分别以体外分离培养的原代搏动的CMs和同代次的hMSCs为阳性对照和阴性对照,利用膜片钳技术观察动作电位。结果在30个诱导分化成心肌样细胞的hMSCs中有6个细胞可诱发出动作电位;CMs具有典型的动作电位;未经诱导的hMSCs没有引出动作电位,5-氮胞苷诱导后细胞可以引出微弱的动作电位。结论实验结果表明hMSCs经5-氮胞苷诱导后,在电学特性方面具有向CMs分化的潜能;与CMs具有的电生理特性相似,这些心肌样细胞具有兴奋性。
Objective To detect whether the cardiomyocyte-like cells differentiated from human marrow mesenchymal stem cells(hMSCs)could produce action potential(AP).Methods Isolated and cultured hMSCs were induced into cardiomyocyte-like cellswith 5-Azacytine in vitro.They were measured for their AP by patch clamp technique,and compared with those of hMSCs of thesatme generation and beating cardiomyocytes(CMs)derived from 2 day-old SD rats.Results 6/30 cardiomyocyte-like cells producedAP.The CMs produced significant AP,hMSCs appeared no AP,and cardiomyocyte-like cells appeared weak AP.Conclusion ThehMSCs manifested the potential to differentiate into CMs in the electrophysiology characteristics following 5-Azacytine induction.
作者
邓方阁
郭连峰
马英智
李玉林
DENG Fang-ge;GUO Lian-feng;MA Ying-zhi(Key Laboratory for Pathobiology of the Ministry of Education,Jilin University,Changchun 130021,Jilin,China;State Key Laboratory of Respiratory Disease,Guangzhou Institute of Respiratory Diseases,the First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510120,China)
出处
《中国康复理论与实践》
CSCD
2009年第11期1017-1018,共2页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家高技术研究发展计划863计划(2004AA205020)
关键词
人骨髓间充质干细胞
心肌样细胞
膜片钳
动作电位
兴奋性
human mesenchymal stem cells(hMSCs)
cardiomyocyte-like cells
patch clamp
action potential(AP)
excitabil-ity
