摘要
穴蛋白(holin)是有尾的双链DNA噬菌体编码的一种跨膜蛋白质,它与内溶素配合对细菌进行裂解。根据拓扑异构学可将穴蛋白分为三大类(第Ⅰ类、第Ⅱ类和第Ⅲ类),它们具有不同的作用特点,但基本作用过程相似,就是在细胞膜上聚集,引起胞膜结构改变,使内溶素穿过细胞膜作用于细胞壁,引起细菌裂解。此外噬菌体还会编码一种对穴蛋白具有抑制作用的抗穴蛋白。抗穴蛋白与穴蛋白相互作用,调节噬菌体对细菌的裂解。
Holin is a membrane protein encoded by double-stranded DNA phage. Holin and endolysin cooperate in host lysis. According to their membrane topology, holins are grouped into three classes class Ⅰ, class Ⅱ and class Ⅲ. They have their own characteristics, but the mechanisms are similar. Holin accumulates and oligomerizes within the membrane, then forms a lesion that permeabilizes the membrane, which leads to bacterial lysis by allowing endolysin to attack the peptidoglycan. And bacteriophage also encodes antiholin, which is a specific negative regulators of holin and can interaction with holin to regulate the bacterial lysis.
出处
《生命的化学》
CAS
CSCD
北大核心
2009年第6期883-887,共5页
Chemistry of Life
基金
国家自然科学基金资助项目(No.30770093)
重庆市自然科学基金资助项目(No.2007BB5066)资助
