摘要
目的观察鼠疫噬菌体对感染鼠疫小鼠的治疗作用,以寻找治疗鼠疫感染的新方法。方法小鼠经皮下感染141株鼠疫菌建立鼠疫感染模型后,用效价为10-11的鼠疫噬菌体对短期治疗组在感染后3、6、12和24 h给予100μl/次的注射治疗,对长期治疗组在感染后1～7 d连续注射鼠疫噬菌体,100μl/次,1次/d。同时,设阴性对照组(不感染,只治疗,观察噬菌体对小鼠的毒性)和阳性对照组(仅感染,不治疗)。结果阴性对照组小鼠治疗14 d后全部存活,处死后剖检未检出鼠疫菌。各治疗组小鼠在治疗第5天存活率为100%,与阳性对照组相比,差异有统计学意义(P<0.05);长期腹腔治疗组效果明显,鼠疫噬菌体在小鼠体内存活时间长,效价不降低。结论鼠疫噬菌体对感染小鼠急性感染期的治疗有一定效果,长期腹腔注射治疗效果较好,为其进一步应用于抗感染治疗提供了实验依据。
Objective To observe the curative effect of plague bacteriophage on Yersinia pestis infection in mice and in-vestigate a novel therapeutic method for plaque.Methods Mouse model of plaque infection was established by subcuta-neous infection with Y.pestis 141 strain.Model mice were treated with 100 μl of plaque bacteriophage at a titer of 10-11 3,6,12 and 24 h after infection to observe the short-term curative effect,or with 100 μl of plaque bacteriophage once a day 1~7 d after infection to observe the long-term curative effect.Uninfected mice were served as negative control to observe the toxicity of bacteriophage,while the infected mice as positive control received no treatment.Results All the mice in negative control group survived 14 d after treatment,in which no Y.pestis was detected.All the survival rates of mice in various test groups were 100% on day 5 after infection,while showed significant difference with that in positive control group（P 〈0.05）.Long-term intraperitoneal injection with plaque bacteriophage showed significantly curative effect,while the bacteriophage survived for a long time and showed no decrease in titer.Conclusion Plague bacteriophage showed a certain curative effect on plague at acute infection stage,of which those by long-term intraperitoneal injection showed satisfactory curative effect.It provided an experimental basis for application of plaque bacteriophage to treatment of Y.pestis infection.
出处
《中国生物制品学杂志》
CAS
CSCD
2013年第4期531-533,共3页
Chinese Journal of Biologicals
