摘要
目的探讨ＡＰＯＥ多态性与血管性痴呆（ＶＤ）和脑梗塞（ＣＩ）的关系。方法应用ＰＣＲ－ＲＦＬＰ技术分析２０例ＶＤ、２４例ＣＩ及２４例健康老年人的ＡＰＯＥ基因型。结果ＶＤ和ＣＩ患者ε３频率均降低（Ｐ＜０．０５），ε４频率均升高（Ｐ＜０．０５），而两组患者间各等位基因频率差异均无统计学意义（Ｐ＞０．０５）；且ε４与血清ＡＰＯＥ、ＡＰＯＢ、ＴＣ、ＬＤＬ－Ｃ正相关，与ＡＰＯＡ、ＨＤＬ－Ｃ负相关。结论ＡＰＯＥ多态性与ＶＤ和ＣＩ的发病机制有关，其在这两种疾病中的作用可能相似。
Objective To detect the gene mutation of apolipoprotein(APOE) in aged patients with vascular dementia (VD) and cerebral infarction (CI),investigate the possible APOE roles in the pathogenesis of these two disorders and the relationship between APOE polymorphism and serum lipid levels.Methods APOE genotypes were analysised by using polymerase chain reaction restriction fragment length polymorphism(PCR RFLP) technique in DNA separeted from peripheral blood of 20 cases of VD,24 cases of CI and 24 sex and age matched control subjects.Serum lipid levels were also measured in all subjects.Results The prevalences of ε 3 allele were 65.00%、64.58%、87.50% and ε 4 allele were 25.00%、22.92%、6.25% in VD、CI and control groups,respectively.The decreased ε 3 allele frequencies(P<0.05) and increased ε 4 allele frequencies (P<0.05) were found in both VD and CI groups,but there was no significant difference between them (P>0.05).ε 4 allele correlated positively with TC、LDL C、APOB and APOE levels and negatively with HDL C and APOA levels (P<0.05,respectively).Conclusion APOE polymorphism has a closed correlation with VD and CI.ε 3、ε 4 alleles may be protective and risk factor,respectively.It could have the similar mechanism in the pathogensis of VD and CI.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
1998年第5期280-282,共3页
Journal of Apoplexy and Nervous Diseases
