摘要
目的探讨热休克蛋白（ＨＳＰ）７０在癫痫发作后的表达及作用。方法用免疫组化及免疫印迹分析法观察红藻氨酸诱导大鼠癫痫发作后ＨＳＰ７０的表达及其动态演变过程。结果正常海马结构含有一定量的组成性ＨＳＰ７０（ＨＳＣ７０），发作后３ｈ即有ＨＳＰ７０表达，２４ｈ达高峰（Ｐ＜０．０１），４８ｈ开始下降，持续至少７ｄ。在２４ｈ，ＨＳＰ７０免疫反应（ＨＳＰ７０－ＩＲ）阳性细胞主要分布在边缘结构。结论ＨＳＰ７０表达是癫痫发作所致细胞损伤的标志，并可能预示细胞死亡或存活。
Objective To study the heat shock protein 70 expression and its role after seizure.Method With immunohistochemical and western blotting method,expression of inducible heat shock protein 70 was observed in rat brain following systemic adminstration of kainic acid,an excitatory neurotoxic analogue of glutamate.Results Kainic acid treatment in rats,showing severe limbic convulsions,caused expression of HSP70 in the rat brain.At 24h,expression was localized in selective cerebral regions,notably the hippocampus CA 1、CA 3 field of Ammon ,s horn,and to a lesser extent,the piriform cortex.We also fould a persistent expression of HSP70 immunoreactivity in neurons for at least 7 days after KA induced seizures.Conclusions These results suggest that HSP70 gives a very high resolution insight into neuronal circuits undergoing prolonged activation and seems to be used as a marker of cellular injury in the rat brain.
出处
《中风与神经疾病杂志》
CSCD
北大核心
1998年第5期277-279,共3页
Journal of Apoplexy and Nervous Diseases
