摘要
在前期获得11个转胆碱脱氢酶基因(betA)小黑杨株系的基础上,将转基因株系与非转基因对照株系在轻度盐碱地上造林,5 a后对试验林的转基因株系进行PCR分子检测,并运用SPSS软件对其树高、胸径、材积和保存率4个生长性状进行了方差分析。结果表明:转betA基因小黑杨外源基因稳定,目前尚未丢失;转基因株系与对照株系间树高、胸径、材积和保存率的差异均达到极显著水平;利用隶属函数法选择的转基因株系T1、T6和T8,为生长速度快、耐盐性高的优良株系。
An afforestation test of transgenie and untransformed control plants was conducted on low-grade salinate fields by means of the obtained 11 betA transgenie Populus simonii × P. nigra plants in the previous work. Five years later, PCR detection for the transgenie plants was done, and variance analysis for tree height, diameter at breast height, volume, and survival rate was also made with SPSS software. Result showed that beta gene still existed in the genome of the transgenic plants. Significant variations were found in tree height, diameter at breast height, volume, and survival rate between the transgenie and untransformed control plants. T1, T6 and T8 transgenic lines were identified as fast growing plants with high saline tolerance by subordinate function.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2009年第11期24-25,28,共3页
Journal of Northeast Forestry University
基金
黑龙江省科技攻关重点项目(GB06B303)资助
关键词
小黑杨
BETA基因
生长性状
耐盐性
Populus simonii × P. nigra
betA gene
Growth characters
Saline tolerances
