摘要
目的探讨ＳＯＤ、ＭＤＡ、ＧＳＨ－Ｐｘ的改变在急性缺血缺氧性脑病（Ｈｙｐｏｘｉｃｉｓｃｈｅｍｉｃｅｎｃｅｐｈａｌｏｐａｔｈｙ，ＨＩＥ）发病机理中的作用。方法将２０只７日龄ＳＤ大鼠随机分为对照组（ｎ＝１０）和急性缺血缺氧性脑病（ｎ＝１０），检测脑组织匀浆中ＳＯＤ、ＭＤＡ、ＧＳＨ－Ｐｘ的含量变化。结果ＨＩＥ模型组病侧脑组织ＳＯＤ及ＧＳＨ－Ｐｘ活力显著下降（Ｐ＜０．００１），ＭＤＡ含量明显提高（Ｐ＜０．００３）。结论ＨＩＥ的发病过程中自由基清除酶类活力下降，脂质过氧化反应增强，提示自由基对急性缺血缺氧性脑损伤的形成起着重要的作用。
Objective To study the free radicals change in the brain of newborn rats with hypoxicischemic encephalopathy (HIE). Methods Twenty newborn Sprague Dawley (SD) rats were randomly divided into normal control group and HIE group.The HIE model was built successively and SOD, GSH Px activity and malonyldialdehyde (MDA) contents in cerebral homogenates were measured.Results In the brain homogenates of HIE rats, SOD content and GSH Px activities were significantly lower than those in the normal control group (P<0.001);the MDA contents showed a higher level (P<0.003).Conclusion HIE could induce the change of free radicals and lead to the injury of brain.
基金
江苏省科委应用基础基金
关键词
SOD
GSH-PX
大鼠
MDA
缺氧缺血性脑病
新生儿
hypoxicischemic encephalopathy
superoxide dimutase(SOD)
glutathione peroxidase
malonyldialdehyde
rats
