摘要
背景与目的:探索建立发生率高、畸形类型明确且易于获得的化学物致小鼠胎鼠短肢畸形的模型的方法。材料与方法:ICR小鼠合笼后,查到阴栓当天为孕期第0天(GD0),孕鼠共50只,随机分为GD9、GD10、GD11、GD12给全反式视黄酸(all-trans-retinoic acid,atRA)实验组和对照组,共5组,每组10只,实验组经口灌胃1次给予孕鼠80 mg/kg atRA,对照组则给予等体积的大豆油,于GD18将孕鼠处死并取出胎鼠,观察不同时间给予孕鼠atRA的胎鼠的短肢畸形情况。结果:于GD10给予孕鼠atRA,可致胎鼠双前肢短小,肱骨、桡骨、尺骨均较正常组短(P均<0.05);于GD11给予孕鼠atRA,可致胎鼠双前肢和双后肢短小,肱骨、桡骨、尺骨、股骨、胫骨均较正常组短(P均<0.05),腓骨常缺失;于GD12给予孕鼠atRA,可致胎鼠尺骨较正常组短(P<0.05)。结论:成功建立了全反式视黄酸致小鼠短肢畸形的模型,为进一步研究肢体畸形的分子机制奠定了基础。
BACKGROUND AND AIM:To develop a chemically-induced,easily established short limb malformation model with high incidence rate and discrete abnormal phenotype.MATERIALS AND METHODS:After the male and female ICR mice copulated,the presence of vaginal plug the following morning was considered as gestational day 0(GD0).At GD9,GD10,GDll and GD12,the pregnant mice in the treatment group were treated with 80 mg/kg all-trans-retinoic acid(atRA),and those of the control group received the same volume of soybean oil.At GD18,pregnant mice were killed and the embryos were harvested.The abnormalities of the embryos in the treatment group were studied.RESULTS:In the group pregnant mice were treated with atRA at GD10,the forelimbs of embryos were short and small,and the humerus,radius and ulna were shorter than those of the control group.In the group treated with atRA at GD11,the forehmbs and the hindlimbs of embryos were short and small,and the humerus,radius,ulna,femur and tibia were shorter than those of the control group,and most of the embryos had no fibula.In the group treated with atRA at GDI2,the ulna of the embryos were shorter than those of the control group.CONLUSION:We developed a model of short limbs induced by atRA during ICR mouse embryogenesis,providing a basis to investigate the molecular mechanism of limb deformity.
作者
王姚立
朱珠
方黎祥
朱勇飞
张天宝
WANG Yao-li;ZHU Zhu;FANG Li-xiang;ZHU Yong-fei;ZHANG Tian bao(Grade 2005,C1inical Medicine,School of Clinical Medicine;Grade 2006,Clinical Medicine,School of Qiajiang;School of Pharmacy and Health Management,Hangzhou Norma l University,Hangzhou 310018,Zhejiang;Department of Health Toxicology,Second Military Medical University.Shangha 200433,China)
出处
《癌变·畸变·突变》
CAS
CSCD
2009年第6期467-470,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
浙江省教育厅计划项目(20061055)
浙江省自然科学基金(Y206360)
关键词
全反式视黄酸
ICR小鼠
胎鼠
短肢畸形
all-trans-retinoic acid
ICR mouse
embryo
short limb malformations
