摘要
目的探讨RARα和β-catenin在过量维甲酸致昆明小鼠腭裂发生中的作用。方法采用免疫组织化学及图像分析方法,检测维甲酸致畸小鼠和相应对照组小鼠腭板中RARα和β-catenin的表达状况。结果实验组RARα在腭板上皮细胞和间充质的表达量明显低于对照组;实验组β-catenin在腭板上皮细胞中的表达量明显高于正常对照组。结论RARα在腭板上皮和间充质细胞中表达的下调和β-catenin在腭板上皮细胞中表达的上调与维甲酸致昆明小鼠腭裂发生相关。
Objective To explore the effects of RARα and β-catenin on cleft palate induced by excess retinoid acid in KM mice. Methods In both malformed embryos induced by excess retinoid acid （the experimental group） and normal KM mice （the control group） the expressions of RARα and β-catenin in the epithelium of palaual shelves and the mesenchyma by immunohistochemistry and image analysis were determined. Results The expression level of RARα in the experimental group was significantly lower than that in the control group, however the expression level of β-catenin were significantly higher in the experimental group than that in the control group. Conclusion The down-regulated expression of RARα in the epithelium of palatal shelves and the mesenchyma and the up-regulated expression of β-catenin in the epithelium of palatal shelves are closely related to the process of cleft palate induced by excess retinoid acid in KM mice.
出处
《山东大学学报（医学版）》
CAS
北大核心
2008年第5期441-444,共4页
Journal of Shandong University:Health Sciences
基金
山东省自然科学基金资助课题(Y2005C63)
关键词
受体
维甲酸
腭裂
Β-链接素
Receptors, retinoic acid
Cleft palate
β-catenin
