摘要
【目的】探讨超高压致死微生物的机理。【方法】本文以副溶血性弧菌为对象,研究了超高压处理对副溶血性弧菌的灭菌效果、对副溶血性弧菌细胞超微结构、细胞无机盐离子含量以及细胞膜蛋白的影响。【结果】结果表明,在20℃下分别经100、200MPa高压处理10min后,副溶血性弧菌致死率为40%、84.7%,经300MPa及以上的压力处理,副溶血性弧菌的致死率为100%。超高压处理对细菌细胞形态结构造成明显的损伤:局部细胞壁遭到破坏,出现缺口;胞质内含物结构紊乱,出现泄漏,细胞中部出现透电子区;细胞结构不完整,细胞膜消失;细菌细胞拟核凝聚和细菌细胞蛋白凝聚。超高压处理破坏了细菌细胞膜蛋白的高级结构,从而对细菌细胞膜的通透性产生了较大影响,引起无机盐等内含物的流失。【结论】研究表明细菌细胞膜的损伤是超高压致死微生物的主要原因,从而为微生物的高压致死机制的研究奠定了理论基础。
[Objective] We explored the mechanism of the microbial inactivation by high hydrostatic pressure. [ Methods] We investigated the effect of ultra high pressure treatment on inactivation of Vibrio Parahaemolyticus cells, and the damages inflicted on the microstructure and the membrane proteins of the Vibrio Parahaemolyticus ceils. [ Results] The result indicated that after pressure treatment at 100 MPa, 200 MPa and 20℃ for 10 min, the viable cell was reduced to 60% and 15.3% respectively, viable cell counts were not detected following pressure treatment at 300 MPa and 20℃ for I0 min. High pressure treatment at 300 MPa also caused changes in the microstructure of the cell, such as the cell wall of Vibrio Parahaemolyticus was shrunk, breached and cytoplasm leaked out, the layer structure of cytoplasm disappeared, and a large electron transmission area appeared in the cell after compression, the membrane disappeared, the nucleoids condensed and the proteins aggregated after compression. At the same time, the membrane proteins of cells were damaged, and the cell membrane permeability increased, resulting in leakage of the cell. [ Conclusion] This study showed that the damaged cell membrane was the major reason of the mechanism of microbial inactivation by high hydrostatic pressure.
出处
《微生物学报》
CAS
CSCD
北大核心
2009年第11期1489-1493,共5页
Acta Microbiologica Sinica
基金
国家“863计划”--加工水产品质量与安全控制技术(2007AA091806)~~
关键词
超高压处理
副溶血性弧菌
细胞膜损伤
ultra-high pressure treatment
vibrio parahaemolyticus
the cell membrane damage
