摘要
为评价肝片吸虫病的细胞免疫反应，选用８头雄性去势水牛和６只雄性山羊，经粪便检查和Ｄｏｔ－ＥＬＩＳＡ检测确认无肝片吸虫感染。经２周适应性饲养后，随机将水牛分成试验组（ｎ＝５）和对照组（ｎ＝３）。试验组水牛每天每头经口感染６０个囊蚴，连续２０ｄ，共接种１２００个囊蚴。山羊分成试验１组、实验２组和对照组，每组２只。试验１组和２组每只山羊分别一次性口服接种５００个和２００个囊蚴。水牛在感染前５周和感染后２６周内，山羊在感染前１周和感染后１６周内，每周颈静脉采血１次，测定外周血Ｔ淋巴细胞增殖反应和／或白细胞介素－２水平。结果表明，水牛在感染后１～６周，Ｔ淋巴细胞增殖反应有上升趋势，而在感染后１３～１７周低于对照组；血清白细胞介素－２水平有类似于淋巴细胞增殖反应的变化。实验１组山羊血清白细胞介素－２水平低于对照组，而实验２组在感染后２～１１周有上升的趋势，以后下降。
Eight testiculectomized water buffaloes and six goat proved to be without fasciolosis by fecal examination and Dot ELISA were used for the experiment to assess their immune response when infected with Fasciola hepatica. After adaptative feeding of two weeks, water buffaloes were randomly divided into infetion group (n=5) and control group (n=3). Each buffalo of infection group orally adminstrated 60 F. heptica metacercariae per day and total 1 200 metacercariae during 20 days per animal. Goats were divided into 3 groups of 2 goats each. The goats in lst and 2nd group were infected with 500 and 200 F. hepatica metacercariae by oral administration, respectively. Periperal lymphocytes proliferation rate and IL 2 concentration were measured weekly. The results showed that lymphocytes proliferation rate in water buffaloes tended to increase during the first 4 weeks of post infection, then declined within the 13th to 17th weeks. Serum IL 2 appeared the similar changes with lymphocyte proliferation. IL 2 level in goats of the infection group 1 was lower than that of control group, but one of the infection group 2 increased fluctuately within the 2nd to llth weeks of post infection, then returned to normal level.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1998年第6期566-568,共3页
Chinese Journal of Veterinary Science
基金
欧盟资助国际合作项目
关键词
肝片吸虫病
水牛
山羊
细胞免疫
asciola hepatica
water buffalo
goat
cellular immune
