摘要
目的:探讨促血管生成素2(Ang-2)在不同程度急性肺损伤(ALI)中的作用。方法:清洁级SD大鼠共40只,随机分为4组:正常对照组,不同剂量大肠杆菌脂多糖(LPS)组(3个剂量组:2 mg/kg、4 mg/kg及8 mg/kg),每组平均10只。HE染色光镜观察肺组织标本病理改变并进行肺损伤评分,Western blot检测各组大鼠血浆中Ang-2的表达情况。结果:LPS可致肺泡间隔增宽、出血及大量炎性细胞浸润等急性肺损伤病理改变,LPS各组的肺损伤评分明显高于正常对照组(P<0.01);LPS不同剂量与肺损伤评分呈量效依赖关系(P<0.05)。LPS不同剂量组的血浆中Ang-2蛋白表达较空白对照组增高(P<0.01),LPS不同剂量与Ang-2蛋白表达水平呈量效依赖关系(P<0.05)。血浆中Ang-2的蛋白表达与肺组织炎症程度积分呈正相关(r=0.862,P<0.05)。结论:Ang-2参与大鼠内毒素性急性肺损伤的病理过程,且血浆Ang-2水平与急性肺损伤程度呈正相关。
AIM： To investigate the potential role of angiopoietin-2 in the rats model of lipopolysaccharide （LPS）- induced acute lung injury （ALI）. METHODS. Wistar rats were randomly divided into four groups （n = 10 ）： control group; in LPS groups, rats were divided into three subgroups, treated with different dosage of LPS （2 mg/kg, 4 mg/kg and 8 mg/kg）. ALl model was established by intravenous injection with LPS, while control group was with NS. The lung histopathology change was observed by HE stain. Western blot was used to measure the expression of Ang-2 in serum. RESULTS. Histologically, alveolar edema, hemorhag, and massive inflammatory cell, infiltration were observed in LPS groups, but not in control group. The pathological score in LPS groups were significantly higher than that in NS group. Compared with 2 mg/kg LPS group, the pathological score in 4 mg/kg LPS group was significantly higher. While the pathological score in 8 mg/kg LPS group was higher than those in other LPS groups. The expression of Ang-2 protein in plasma was significantly higher in LPS groups, but weak in control group. Compared with 2 mg/kg LPS group, the expression of Ang-2 protein in 4 mg/kg LPS group was significantly higher. While the expression of Ang-2 protein in 8 mg/kg LPS group was higher than those in other LPS groups. The expression of Ang-2 protein in plasma were significantly positively correlated with the pathological score （r=0.862, P〈0.05）. CONCLUSION： Ang-2 could participate the pathological course in the rats model of LPS-induced acute lung injury. The expression of Ang-2 protein in serum was significantly positively correlated with the extent lung injury.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2009年第11期1005-1007,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
重庆市科委自然科学基金资助课题(2003-7673)
