摘要
以辽河流域12个不同断面的河水为诱变剂,运用蚕豆根尖微核检测技术和染色体畸变实验方法,测定蚕豆根尖细胞的有丝分裂指数、微核率和染色体畸变率。结果表明:不同断面的河水均能降低蚕豆根尖细胞有丝分裂指数,能诱发较高频率的微核和染色体畸变,产生染色体断片、核突起和核碎裂。所有样点微核率和染色体畸变率均高于对照组。根据采样点水质污染指数分析可知,福德店水质属重度污染,东辽河、条子河、红庙子水质属中度污染,招苏台河水质属轻度污染。
Water in 12 sections of Liao River Valley was sampled as mutagen and micronucleus test and chromosome aberration assay were used to detect mitotic index, micronucleus ratio and chromosome aberration ratio of root tip cells in broad bean ptant （Viciafaba）. Results showed that water in different sections can decrease the mitotic index in Viciafaba root tip cells, induce higher ratios of either micronucleus or chromosome aberration and produce chromosomal segments, nuclear protuberances and fragments. Micronucleus ratio and chromosome aberration ratio in different sections were higher than those of the control group. Analysis of pollution index in 12 sections showed that water quality was graded as heavy pollution in Fudedian River, moderate pollution in Dongliao River, Tiaozi River and Hongmiaozi River and light pollution in Zhaosutai River.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2009年第12期35-38,91,共5页
Environmental Science & Technology
基金
国家水体污染控制与治理科技重大专项(2008ZX07208-001)
关键词
辽河
蚕豆
微核
染色体畸变
Liao River Valley
broad bean plant （ Vicia faba ）
micronucleus
chromosome aberration
