摘要
目的：研究蒙古黄芪中不同提取部位的抗氧化活性。方法：采用生物化学发光法对蒙古黄芪的总黄酮、总皂苷以及总多糖于1.25×10-3-25×10-3g.L-1的浓度检测其抗氧化能力。结果：蒙古黄芪的不同提物部位对3种自由基均有清除作用,而且都呈明显的量效关系。其中,总黄酮清除自由基的能力最强,不同纯度的总黄酮（55.48%TFA,0.59%TFA和0.36%TFA）清除超氧阴离子的IC50分别为0.654 2,4.654 4,6.055 2 g.L-1,清除羟基离子的IC50分别为0.060 5,0.254 4,0.493 0 g.L-1,清除过氧化氢的IC50分别为0.010 4,0.156 0,0.224 0 g.L-1。结论：蒙古黄芪对过氧化氢、羟基离子的清除能力明显强于超氧阴离子。总黄酮是蒙古黄芪抗氧化活性的主要成分,总皂苷和总多糖的抗氧化能力较弱。总黄酮清除自由基的能力随纯度的增高而增高,相反,总皂苷、总多糖清除自由基的能力随纯度的增高而下降。
Objective： To study the antioxidant activities of,different extracts from Astragalus mongholicus. Method： The antioxidant activities of the total flavanoids （ TFA）, the total saponins （TSA） and the total polysaccharides （TPA） from A. mongholicus were measured by means of biochemiluminescence. The concentrations of these samples varied from 1.25 × 10^-3g · L^-1 to 25 ×10 ^-3 g· L^-1. Result： All the extracts showed antioxidant activities of scavenging 3 different free radicals with obvious concentration-scavenging capacity relationship. The antioxidant activity of TFA was the strongest among them. The IC50 of scavenging superoxide anion radicals of TFA （55.48%TFA, 0. 59%TFA and 0. 36%TFA） were 0. 654 2, 4. 654 4 and 6. 055 2 g· L^-1, the IC50 of scavenging hydroxyl anion were 0. 060 5, 0. 254 4 and 0. 493 0 g · L^-1 , and the IC50 of scavenging hydrogen peroxide were 0. 010 4, 0. 156 0 and 0. 224 0 g · L^-1, respectively. Conclusion： The extracts are more sensitive to hydrogen peroxide and hydroxyl anion than to superoxide anion radicals. TFA of A. mongholicus Bunge is the main component responsible for antioxidant activity, while TSA and TPA have weaker antioxidant activity. The antioxidant activity of TFA increases with the rise of its purity. On the contrary, the antioxidant activity of TSA and TPA decreases with the rise of their purity.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2924-2927,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30472160)
关键词
蒙古黄芪
抗氧化
提取部位
Astragalus mongholicus
antioxidant
extracts
