摘要
目的：建立白屈菜药材的质量标准。方法：采用甲醇提取,高效液相色谱法测定,以乙腈-1%三乙胺溶液（25∶75）（三乙胺溶液用磷酸调节PH至3.0）为流动相;检测波长268 nm。结果：白屈菜红碱进样量在0.051 6-0.516 0μg线性关系良好,r=1.000,平均加样回收率（n=6）103.0%,RSD 1.2%。供试品溶液在8 h内稳定,用不同色谱柱分析耐用性好。结论：所建立的分析方法简单、专属性强,结果可靠、准确。
Objective： To develop an HPLC method for determination of the content of chelerythrine in Chelidonium majus. Method： Chelerythriue was extracted from the fine powder of the crade with drug methanol and determined by HPLC. The mobile phase was acetonitrile-1% triethylamine （25：75 ） （ adjusted pH to 3 using phosphoric acid） and the detection wavelength was set at 268 nm. Result： The linear range of calibration curve was 0. 051 6-0. 516 0 μg （r = 1. 000）. The average recovery （n =6） was 103.0% with RSD of 1.2%. Chelerythrine in the sample solution was stable in 8 h and the ruggedness was perfect among 3 different chromatographic columns. Conclusion： The method is accurate, sensitive and reliable.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2918-2919,共2页
China Journal of Chinese Materia Medica
