摘要
目的：建立佩兰药材中石竹烯含量的测定方法。方法：采用溶剂超声提取样品、气相色谱法[DB-1701弹性毛细管柱（0.32 mm×30 m,0.25μm）]测定佩兰药材中石竹烯的含量。结果：石竹烯在0.002-2.0 g.L-1呈良好线性关系（r=0.999 9）,加样回收率在96.8%-104.2%。结论：本方法准确可靠、简便、重复性好,可用于佩兰的质量控制。
Objective： To develop a GC method to determine the content of caryophyllene in Eupatoriumfortune. Method： The samples were determined on a DB-1701 （0. 32 mm ×30 m, 0. 25μm） quartz capillary column. And the sample was extracted with ethanol by the ultrasonic assisted extraction. Result： The calibration curve of caryophyllene is liner over the range of 0. 002-2. 0 g ·L ^-1 （R2 = 1 ）. The recovery was from 96.76% to 104. 15%. Conclusion： The method is accurate, simple with a good reproducibility. It can be used to control the quality of E. fortune.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2009年第22期2907-2909,共3页
China Journal of Chinese Materia Medica
基金
<中国药典>2010年版一部标准研究(YS-209)
关键词
佩兰
石竹烯
气相色谱法
含量测定
Eupatorium fortune
main constituent
GC
determination
caryophyllene
