摘要
为了探明猪SP-A基因的启动子及其转录调控机制,设计6条特异性引物,采用染色体步移、克隆测序以及序列比对分析,从大白猪基因组中扩增出一段长度为1 033 bp的猪SP-A基因的上游序列(DQ985806),其GC含量约为55%。经过生物信息学分析,确定了其转录起始位点及活性区域;发现在转录起始位点上游-33 bp处存在1个TATA-box,另外还发现了GC-box、CAAT-box、GT-box以及转录起始子;该序列还包含Sp1、TF和NF-κB等转录因子结合位点以及1个特殊重复序列5-′CATCACTGT-3′。上述试验结果表明,所克隆的1 033 bp的DNA序列是大白猪SP-A基因的启动子区序列。
In order to explore the mechanism of porcine SP-A gene transcription regulation by studying the promoter region of porcine SP-A gene,the upstream sequence of Large White porcine SP-A gene was attained by Genome Walking PCR,sequencing and sequences alignment analysis.The DNA sequence of 1 033 bp（DQ985806） was amplified.The ratio of G and C in the sequence was about 55%.The sequence analysis showed that there were a TATA-box at-33 bp and GC-box,CAAT-box,GT-box and transcriptional initiator.Meanwhile,some transcriptional factor binding sites including Sp1,TF,NF-κB,and so on were detected.In addition,a special repetitive sequence of 5′-CATCACTGT-3′ was also detected.The results showed that the cloned sequence（1 033 bp） may be considered as the promoter region of Large White porcine SP-A gene.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第11期1600-1608,共9页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家科技支撑计划项目(2006BAD01A08
2007BAD51B02)
山西省科技攻关项目(052011)
关键词
猪SP-A基因
启动子区
克隆
染色体步移
序列分析
porcine surfactant protein A gene
promoter region
clone
Genome Walking
sequence analysis
