摘要
目的观察益肾蠲湿合剂对肾小球系膜细胞(glomerular mesangial cell,GMC)增殖及细胞外信号调节激酶(extracel-lular signal-regulated kinases,ERK)磷酸化的抑制作用。方法将含低、中、高剂量益肾蠲湿合剂的药物血清作用于10%血清-DMEM培养液培养的GMC,分别用MTT和Western Blot方法检测细胞增殖和磷酸化ERK1/2的表达。结果益肾蠲湿合剂对GMC增殖有明显的抑制作用,并随着剂量的增加作用也逐渐增强。细胞总ERK在各组间无明显差别。但不同浓度的益肾蠲湿合剂均显著地抑制血清所诱导的ERK1/2的磷酸化。结论益肾蠲湿合剂可通过抑制ERK1/2的磷酸化抑制GMC的增殖。
Objective To observe the effect of ＂Yishenjuanshi＂ on the proliferation of glomerular mesangial cells（GMCs） and the phosphorylation of extracellular signal-regulated kinases（ERK）.Methods All herbs in ＂Yishenjuanshi＂ mixture were boiled in water and concentrated to different doses.After 7 days of ＂Yishenjuanshi＂ treatment,serum from rats eye blood was collected.GMCs were cultured in serum free medium for 48hs and then 10% FSC-DMEM with or without serum including ＂Yishenjuanshi＂ to detect GMCs proliferation and expression of phosphorylated ERK （ p - ERK） by MTF and Western Blot respectively. Results Low, medium and high dose of Yishenjuanshi＂ treatment significantly inhibited GMC proliferation when GMCs were cultured for 24, 48 and 72 h ; and the inhibition ratios increased as the dose increased. There was no difference in total ERK expression. However, ＂Yishenjuanshi＂ treatments with different dose all markedly inhibited p - ERK expression stimulated by FCS. Conclusion ＂Yishenjuanshi＂ treatment inhibits GMC proliferation via blocking ERK phosphorylation.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第11期2722-2724,共3页
Lishizhen Medicine and Materia Medica Research
基金
河北省唐山市科学技术局资助(No.04362001B-12)
