摘要
目的 :探讨肾康注射液 (SKI)阻抑肾小球硬化 (GS) ,防治慢性肾功能衰竭 (CRF)的部分作用机制 ,为SKI临床应用提供依据 .方法 :采用血清药理学实验法 ,观察SKI对体外培养人肾小球系膜细胞 (GMC)增殖及其自分泌纤维连接蛋白(FN)水平的影响 .结果 :SKI及其含药血清对GMC增殖、自分泌FN有明显抑制作用 (P <0 .0 1,P <0 .0 5 ) ,呈剂量或SKI血药浓度与效应依赖性 ;但随刺激时间延长 ,其作用存在差异 ,即对增殖的抑制效应 :SKI呈递增趋势、而SKI含药血清则呈递减趋势 ,对FN水平的抑制效应则相反 .结论 :SKI经机体代谢 ,药理效应的不一致性 ,提示SKI含药血清可能出现提高FN降解酶活性的新物质 。
AIM: To explore the mechanism of ShenKang Injection (SKI) on prohibiting glomerulosclerosis (GS) so as to provide fresh evidence for clinical application of SKI in treating chronic renal failure (CRF). METHODS: Using serum pharmacological test of Chinese herbs, the effects of SKI on the proliferation in cultured human glomerular mesangial cells (GMC) and the level of fibronectin (FN) secretion in cultured GMC were observed. RESULTS: SKI and serum with SKI significantly inhibited the proliferation and the level of secreting FN in cultured GMC in a concentration dependent manner ( P<0.01, P <0.05). With the prolonging of stimulating time, the inhibitory effects SKI increased on the proliferation and the level of FN, but the effects of serum with SKI decreased on the proliferation and the level of FN. CONCLUSION: The effects of SKI on GMC differ from those of serum with SKI. It is indicated that the effective mechanism of SKI is complicated and is related to SKI metabolism.
出处
《第四军医大学学报》
CAS
北大核心
2003年第5期433-435,共3页
Journal of the Fourth Military Medical University
