摘要
为研究血啉甲醚（ＨＭＭＥ）与小鼠血浆的蛋白结合率及ＨＭＭＥ与血浆蛋白的结合参数，用平衡透析法测定蛋白结合率，ＨＭＭＥ浓度用标准曲线法求出。ＨＭＭＥ血浆浓度在１９４μｇ／ｍｌ～２９７６μｇ／ｍｌ时血浆蛋白结合率为９３５％～７６３％，血浆蛋白结合参数：最大结合力（β）为８７×１０－６ｍｏｌ／ｇ；解离常数（Ｋｄｐ）为４２４×１０－５ｍｏｌ／Ｌ；结合常数（Ｋ）为２８４×１０４Ｌ／ｍｏｌ；结合部位数（Ｎ）为１４９２。表明ＨＭＭＥ与小鼠血浆蛋白具有单一类型的结合部位，药物血浆浓度升高结合率下降。
o elucidate the binding of hematoporphyrin monomethyl ether (HMME) to plasma protein of mice and evaluate the binding parameters, the binding rate of HMME to plasma protein was determined by equilibrium dialysis method. Concentration of HMME was assayed by standard curve method. The binding rate of HMME to plasma protein was found to be 93.5%-76.3% varying with the concentration of HMME in plasma from 19.4 μg/ml-297.6 μg/ml. The binding parameters were 8.7×10^-6mol/g for β; 4.24×10^-5mol/L for Kdp; 2.84×10~4 L/mol for K and 1.492 for N. There were singletyped binding sites for HMME to plasma protein of mice. Moreover plasma concentration of HMME was inversely proportional to the binding rate.
出处
《中国激光医学杂志》
CAS
CSCD
1998年第3期152-155,共4页
Chinese Journal of Laser Medicine & Surgery
关键词
血啉甲醚
血浆蛋白结合率
结合参数
平衡透析法
Hematoporphyrin monomethyl ether, Plasma protein binding rate, Binding parameters, Equilibrium dialysis
