摘要
目的研究新生儿胎盘中印记基因H19和胰岛素样生长因子Ⅱ基因(IGF-Ⅱgene)mRNA的表达,探讨印记基因H19和IGF-Ⅱ与出生体质量的关系。方法收集无妊娠期并发症及无胎盘脐带异常的足月儿新鲜胎盘共30例,其中正常出生体质量儿12例、巨大儿10例,足月小样儿8例,采用实时荧光定量PCR方法检测以上标本中H19和IGF-ⅡmRNA表达。结果H19基因在胎盘中表达与出生体质量呈负相关(r=-0.403,P=0.027),IGF-Ⅱ基因在胎盘中表达与出生体质量呈正相关(r=0.444,P=0.014);巨大儿组胎盘中H19mRNA表达(0.21±0.31)较足月小样儿组(1.51±2.04)明显减少(P=0.013);巨大儿组胎盘中IGF-ⅡmRNA表达(2.67±3.41)较足月小样儿组(0.39±0.33)明显增加(P=0.013)。结论印记基因H19与IGF-Ⅱ的表达在不同出生体质量儿胎盘中差别明显,两基因与出生体质量的影响机制相关,且两基因之间存在一定相互关系。
Objective To study the expression of the imprinted gene H19 and IGF- Ⅱ in newborn placenta,and to discuss its influence on the birth body mass of the neonate. Methods The fresh placental tissues from full-term newborn （without trimester of pregnancy complication and placenta and funic abnormality） with normal,high and low birth body mass （12,10 and 8 samples respectively）were collected. The expression of imprinted gene H19 and IGF-Ⅱ mRNA in the placenta were estimated by real-time fluorescence quantitative PCR. Results The expression of H19 mRNA in the placenta was negative correlation to the birth body mass （r =-0.403 ,P = 0.027）.The expression of of IGF-Ⅱ mRNA was positive correlated to the birth body mass （r = 0.444,P = 0.014）. The H19 mRNA expression level in the high birth weight neonates （0.21±0.31） was significantly lower than that in the low birth body mass neonates （ 1.51±2.04）（P = 0.013 ）. But the expression level of IGF-Ⅱ mRNA in the high birth body mass neonates （2.67 ±3.41 ） was significantly higher than that in the low birth body mass neonates （0.39±0.33）（P =0.013）. Conclusion The expression of H19 and IGF-Ⅱ mRNA was significantly different in the placenta of normal,high and low birth body mass newborns. These two genes may be related to the birth body mass,and there may be some realationship between these two genes.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2009年第10期758-760,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(30672237)
