摘要
研究了镧(La3+)和铕(Eu3+)对蚕豆保卫细胞外向钾通道(K+out)电流的作用.在细胞外,La3+对K+out电流是抑制的,半抑制浓度EC50为81μmol·L-1;Eu3+对K+out电流表现为低浓度(10和50μmol·L-1)激活,高浓度(≥1mmol·L-1)抑制.La3+在细胞内对K+out电流也表现为低浓度(1.13×10-14mol·L-1)激活,高浓度(5.86×10-14mol·L-1)抑制现象.稀土离子La3+和Eu3+对保卫细胞K+out电流的作用,可能和植物调节水分有关,这可能是稀土生物效应的机制之一.
Effects of La^3＋ and Eu^3＋ on outward potassium channels （Kout^＋） in Vicia guard cells have been studied by patch clamping technique. Extracellular La^3＋ inhibited Kout^＋ currents with a half-inhibition-concentration （EC50） of 81 μmol·L^-1. Interestingly, intracellular La^3＋ activated Kout^＋ currents at a free concentration of 1.13 ×10^-14 mol·L^-1, and inhibited Kout^＋ currents at a free concentration of 5.86×10^-14 mol·L^-1. Extracellular Eu^3＋ also activated Kout^＋ currents at concentrations of 10 μmol·L^-1 and 50μmol·L^-1, and inhibited Kout^＋ currents at concentrations of more than 1 mmol·L^-1. The effects of La^3＋ and Eu^3＋ on Kout^＋ currents may have function to regulate plant water status, which may be one of the mechanisms of biological effect of rare earth elements.
出处
《中国科学（B辑）》
EI
CSCD
北大核心
2009年第11期1363-1367,共5页
Science in China(Series B)
基金
国家自然科学基金(批准号:20701028)资助
关键词
稀土
气孔
保卫细胞
外向钾通道
rare earth elements, stomata, guard cell, outward potassium channel
