摘要
目的:探讨脆性组氨酸三联体(FHIT)基因转染对人胃癌细胞MKN-45迁移和侵袭能力的影响。方法:将载有人外源性FHIT基因的真核表达质粒pcDNA3.1-FHIT,转染至FHITmRNA阴性表达的人胃癌细胞MKN-45,分别应用Millicell小室细胞迁移实验、Transwell小室侵袭实验检测转染前后MKN-45细胞迁移能力和侵袭力的改变。结果:外源性FHIT基因转染MKN-45细胞后,MKN-45细胞的迁移能力明显降低(P<0.05),但细胞侵袭能力无明显变化(P>0.05)。结论:外源性FHIT基因转染致MKN-45细胞系的迁移能力明显降低,对MKN-45细胞系的侵袭能力无明显影响。
Objective To investigate the effects of fragile histidine triad （FHIT） gene on migration and invasion of MKN-45 human gastric cancer cell line. Methods Eukaryotic expression carrier of recombinant pcDNA3.1-FHIT was transfected into human gastric cancer cell line MKN-45 by liposome. Migration assay was adopted to test whether FHIT was involved in the migration of MKN-45 cells in vitro. We examined the invasiveness of MKN-45 cells before and after transfected by invasiveness test in vitro. Results The recombinant plasmid pcDNA3.1 was transfected into MKN-45, which made FHIT highly expressed in FHIT-regenerating cell line MKN-45. In vitro, Millicell assay showed that the quantity of MKN-45 threated with pcDNA3.1-FHIT was markedly decreased compared with control MKN-45 cells. There were no significant differences on invasiveness in MKN-45 cells with and without transfected. Conclusions The pcDNA3.1-FHIT has been transfected successfully, which makes FH1T highly expressed in FHIT-regenerating cell line MKN-45. FHIT gene may inhibit MKN-45 cell migration. FHIT gene is a potential suppressor gene and play an important role in gastric cancer mechanism.
出处
《东南大学学报（医学版）》
CAS
2009年第5期397-401,共5页
Journal of Southeast University(Medical Science Edition)
基金
右江民族医学院科研基金资助项目
关键词
脆性组氨酸三联体
胃癌
基因转染
迁移
侵袭
fragile histidine triad gene
gastric cancer
transfection
migration
invasion
