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FHIT基因过表达对人胃癌细胞系MKN-45增殖和粘附的影响 被引量：1

FHIT gene over-expression impacts on proliferation and adhesion of human gastric cancer cell line MKN-45
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摘要 目的探讨FHIT基因过表达对人胃癌细胞系MKN-45增殖和粘附能力的影响。方法将载有人外源性FHIT基因的真核表达质粒pcDNA3.1-FHIT转染FHIT基因mRNA阴性表达人胃癌细胞系MKN-45,分别用MTT法、平板克隆形成实验、黏附实验检测转染前后细胞增殖活性、克隆形成能力及粘附能力的变化。结果FHIT基因的过表达可降低MKN-45细胞的增殖和克隆形成能力(P<0.05),但是对MKN-45细胞与内皮细胞的粘附能力无明显影响。结论FHIT基因过表达可抑制人胃癌细胞系MKN-45的增殖和克隆形成,对于MKN-45细胞与内皮细胞的粘附能力无明显影响。 Objective To evaluate the effects of FHIT gene over - expression on human gastric cancer cell line MKN-45 proliferation and adhesion. Methods Human xenogenous eukaryotic expression plasmid pcDNA3.1 -FHIT were transfected to mRNA - negative human gastric cell line MKN - 45. A MTT assay, plate clony - forming test and adhesion test were performed to assess the MKN- 45 cells＇ proliferative activity, clony- forming capability and adhesive ability before and after transfection. Results FHIT over - expression can reduce MKN - 45 cells＇ proliferation and clony- forming capability （ P 〈 0.05）, but it was ineffectiveness on MKN- 45 cells and endothelial cells adhesion. Conclusion FHIT over- expression can inhibit the proliferation and clony- forming ability of human gastric cell line MKN - 45, it dose not work on MKN - 45 cells and endothelial cells adhesive ability.
作者 黄照权 刘飞 黄培林 李楠 徐佳佳 吴鹏
机构地区 右江民族医学院附属医院 东南大学附属南京第二医院肿瘤科 东南大学医学院病理学系
出处 《右江民族医学院学报》 2009年第2期155-157,共3页 Journal of Youjiang Medical University for Nationalities
基金 右江民族医学院科研基金项目
关键词 FHIT 胃肿瘤 细胞增殖 克隆 分子 基因 转染 细胞粘附 FHIT gastric neoplasms cell proliferation cloning, molecular genes transfection cell adhesion
分类号 R735.2 [医药卫生—肿瘤]
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右江民族医学院学报
2009年 第2期

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