摘要
目的筛选大孔树脂吸附法分离丹酚酸的最佳条件。方法采用动态吸附-解吸方法,用HPLC法测定丹酚酸B的含量对工艺进行评价。结果采用ZTC-1型大孔吸附树脂,上柱量按每毫升树脂吸附7.95mg丹酚酸B计算,以4倍柱体积水洗脱;收集30%乙醇洗脱液,约为6倍柱体积。总丹酚酸得率为7.9%,含丹酚酸B为77.8%。结论该法简单可行,分离效果好,可为工业生产提供参考数据。
Aim To study the optimal separation of the salvianolic acids from dan-shen root with appropriate macroporous resin. Methods Dynamic adsorption-desorption methods were adopted and evaluated for separation efficiency by measuring the concentration of salvianolic acid B with HPLC. Results The refining process condition was as follows： lmL of sorbent absorbed 7.95 mg of salvianolic acid. 4BV （ resin bed volume） water and 6 BV 30% ethanol were used as eluation. Then the 30% ethanol eluation was selected and concentrated under reduced pressure. The yeild was 7.9%. The content of salvianolic acid B 77.8% of the total salvianolic acids. Conclusion This method is simple and feasible with good effect of separation, which can offer reference for industrial production.
出处
《解放军药学学报》
CAS
2009年第5期439-441,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
关键词
丹酚酸
大孔树脂
丹酚酸B
salvianolic acids
maeroporous resin
salvianolic acid B
