摘要
目的观察苯丙氨酸二肽类化合物190对四氯化碳致小鼠急性肝损伤的保护作用。方法将180只小鼠随机分为正常组,CC l4模型对照组,联苯双酯阳性药物组和PDC190低、中、高(25、50、100mg.kg-1)3个剂量组。应用0.12%CC l4腹腔注射致小鼠急性肝损伤模型,通过测定小鼠血清中谷丙转氨酶、谷草转氨酶活性以及小鼠肝组织中丙二醛含量和超氧化物歧化酶活力,并观察肝脏组织病理学变化,对PDC190的保肝作用进行研究。结果模型组小鼠肝功能明显异常,肝组织变性、坏死严重;PDC190组能明显改善CC l4中毒小鼠肝功能,其降酶率以低、中、高剂量分别为22.1%、70.2%、78.5%;肝组织切片镜检证实,苯丙氨酸二肽类化合物190能减轻中毒小鼠肝细胞损伤程度。结论苯丙氨酸二肽类化合物190对急性肝损伤小鼠有较好的保肝作用。
Aim To observe the protective effect of phenylalanine dipeptide compounds 190（PDC190） on mouse models of CCl4 acute liver injury. Methods 180 mice were randomly divided into the normal group, bifendate control group, CCl4 model group and three PDC190 groups[ high （ 100mg·kg^-1 ）, middle （50mg·kg^-1 ） and low （25mg·kg^-1） dosage group ] in the experiment. Acute hepatic injury models of mice were induced by 0.12% CCl4 through intraperitoneal injection. The protective liver effect of PDC190 was observed by measuring ALT,AST activity in serum, MDA content and SOD activity in hepatic tissues. Liver histopathological changes were also observed. Results In the model group, the level of ALT, AST increased significantly , and hepatic tissue degeneration was obviously seen. In PDC190 groups ,the level of ALT dropped significantly（ the restrain rate of the high ,middle and low dosage group was 78, 5% ,70.2% ,22.1% respectively）. Liver histological section test under a microscope proved that PDC190 could relieve the degree of hepatic injury of mice induced by CCl4. Conclusion PDC190 has good protective effect on mice with acute liver injury.
出处
《解放军药学学报》
CAS
2009年第5期383-386,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家科技部"国际合作"新药基金项目
No.2008DFB30110
国家自然科学基金项目
No.30760292
贵州省中药现代化项目
No.(黔科合农字[2006]5001号)
973计划前期研究专项资助
No.2007CB516800
