摘要
目的:为研究制备一种骨组织工程支架材料奠定基础。方法:用猪椎骨为原料,低温锻烧后制得主要成分为羟基磷灰石(HA)的陶瓷化骨(TBC)。将TBC与焦磷酸钠溶液(Na4P2O7.10H2O,NP)混合再次锻烧,制得以磷酸三钙(TCP)和HA为主要成分的双相陶瓷生物骨(BCBB),并观察其结构、检测其成分和抗压强度。结果:BCBB呈白色,为天然网孔状结构,孔径范围462±123μm,孔隙率为59.5±2.5%。蛋白和氮元素含量为0,BCBB主要由79.1%HA和17.4%TCP组成,钙磷原子数量比为1:1.59±0.11,抗压强度应为.30±0.68MPa。结论:该研究制备的BCBB有望成为一种理想的骨组织工程支架材料。
Objective： To establish foundation for the study of a scaffold of tissue engineering bone. Method： The swine vertebral cancellous bone with cortex on one side was calcined to make true bone ceramic （TBC） which was mainly made up of hydroxyapatite（HA）, and then biphasic ceramic biologic bone （BCBB） was fabricated by mixing TBC with the aqua of Na4P2O7 ·10H2O （NP） and calcined this composite once again, which was mainly made up of hydroxyapatite（HA）and tricalcium phosphate（TCP）. At the same time, a series of examinations were carried out including of scanning electron microscope （SEM）, energy dispersion analysis X-ray（EDAX）, and biomechanics examination, et al. Result： BCBB was white and its surface was uneven. The pore size of BCBB is 462±123μm. The average porosity is 59. 5±2. 5μm. BCBB was mainly composed of hydroxyapatite （79. 1%） and tricalcium phosphate（17.4%）. The Ca/P element ratio of BCBB is 1：1.59±0. 11. The anti-press strength was （3. 30±0. 68）MPa. The protein content of BCBB was zero and the organic substance had been completely done away with. Conclusion： It is hopeful to this BCBB become a scaffold of tissue engineering bone.
出处
《陕西医学杂志》
CAS
2009年第11期1470-1472,共3页
Shaanxi Medical Journal
基金
贵州省优秀科技教育人才省长基金项目(黔省专合字200768)
贵州省科学技术基金项目(黔科合J字20072104)
云南省教育厅科学研究基金项目(5J0348C)
