摘要
目的:探讨三七对酒精性肝病大鼠体内SOD和MDA的影响。方法:SD大鼠随机分为正常组、模型组、三七高、低剂量组和硫普罗宁组,均连续14周建立酒精性肝病模型。在模型制备的同时,每天下午灌服给药。黄嘌呤氧化酶法测定SOD,硫代巴比妥酸法测定MDA。结果:模型组大鼠血清及肝组织SOD水平较正常组降低(P<0.05),三七高、低剂量组大鼠血清及肝组织SOD水平较模型组升高(P<0.05),模型组大鼠血清及肝组织MDA水平较正常组升高(P<0.05),三七高、低剂量组大鼠血清MDA水平较模型组降低(P<0.05)。结论:三七可提高ALD大鼠肝组织及血清SOD活性、降低MDA含量,抑制氧应激和脂质过氧化反应。
Objective：To investigate the effects of Notoginseng on SOD activities and MDA contents in serum and liver of of alcoholic hepatopathy rats. Methods ： SD male rats were randomly divided into five groups ： normal group, control group, high -dose Notoginseng group, low -dose Notoginseng group, and Tiopronin group. All rats were induced alco- holic hepatopathy in 14 weeks and were treated every afternoon during the 14 - week respectively. The SOD of blood and liver hepatic tissue were determined by xanthine oxidase enzymic method. The MDA of blood and liver hepatic tissue were determined by thio - barbituric acid method. Results ： Compared with rats from the normal group, rats in the control group have lower of SOD in blood and liver hepatic tissue （P 〈 0.05 ）, but treatment with high - dose, low - dose Notoginseng, or Tiopronin resulted in higher level of SOD（P 〈 0.05 ）. Compared with rats from the normal group , rats in the control group have higher level of MDA in blood and liver hepatic tissue（ P 〈 0.05 ）, but treatment with high - dose, low - dose Notoginseng, or Tiopronin resulted in lower level of MDA（ P 〈 O. 05 ）. Conclusion ： Sanchi can significantly degrade the level of MDA and advance the level of MDA in the alcoholic hepatopathy rats. So Sanchi can inhibit oxidative stress and lipid peroxidation.
出处
《中华中医药学刊》
CAS
2009年第11期2387-2390,共4页
Chinese Archives of Traditional Chinese Medicine
基金
浙江省中医药科技计划项目(2006Y018)
杭州市医药卫生科技计划项目(2005B071)
关键词
酒精性肝病
三七
超氧化物歧化酶
丙二醛
alcoholic liver diseases
Notoginseng
superoxide dismutase
malondial dehyde
