摘要
综述了溶剂提取法、酶提取法、超声波与微波辅助提取法在植物多糖提取中的应用;分析了膜分离法、有机溶剂萃取法、柱层析法等分离纯化植物多糖方法的现状和前景;总结了苯酚-硫酸法、蒽酮-硫酸法、DNS(3,5-二硝基水杨酸)比色法、咔唑-硫酸比色法、高效凝胶渗透色谱法(HPGPC)等植物多糖的含量测定方法。植物多糖的分离纯化与含量分析,既是进一步研究其分子结构的基础,也为制备工业化产品提供参考。
The research progress on extraction, purification and content determination of plant polysaccharides were reviewed. Extraction methods such as solvent extraction, enzyme extraction, microwave and ultrasound-assisted extractions were summarized. The separation and purification methods were discussed, including membrane separation, organic solvent extraction, column chromatography, etc. The principle and application of content determination methods covering phenol-sulphuric acid method, anthrone-sulphuric acid method, 3,5-dinitrosalicylic acid （DNS） method, carbazole-sulfuric acid colorimetric method, HPGPC method, etc. were also reviewed. The study on the extraction, separation and determination of plant polysaccharides will establish the basis of further research of plant polysaccharide＇s molecular structure and provide references for the exploitation of related industrial products.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2009年第B10期238-242,共5页
Chemistry and Industry of Forest Products
基金
"十一五"国家科技支撑计划资助(2009BADB1B03)
湖南省科技计划项目(2009NK3123)
关键词
植物多糖
分离纯化
含量测定
plant polysaccharide
purification
content determination
