摘要
目的:建立环介导恒温扩增快速检测肠毒素性大肠埃希菌的LTⅠ毒素的方法。方法:采用环介导恒温扩增反应(LAMP)技术扩增肠毒素性大肠埃希菌的LTⅠ毒素特异性基因,优化反应条件,并与传统PCR比较。结果:与传统的PCR技术相比,LAMP法更加简便快速,且在等温条件下进行,具有更高的灵敏性及特异性。结论:该方法灵敏度高,特异性强,操作简便快速,不需要复杂仪器设备,为检测产肠毒素性大肠埃希菌的LTⅠ毒素提供了一个快速简便的新方法。
Objective：To develop a technique for detection of LT Ⅰgene of enterotoxigenic Echerichia coli（ETEC） using a novel DNA amplification procedure designated Loop-mediated isothermal amplification（LAMP）.Methods：The heat-liable Ⅰ enterotoxin genes of ETEC was amplified by LAMP,and compared with PCR.Results：Compared with PCR,the LAMP method was performed under isothermal conditions.Its specificity was 100% and the sensitivity was 10-fold higher than that of conventional PCR.Conclusion：This LAMP-based assay is simple,rapid,sensitive and specific.No specialized equipment is needed which makes it is more economical and practical.This assay is expected to become a valuable tool for rapid diagnosis in ETEC infection.
出处
《中国卫生检验杂志》
CAS
2009年第11期2572-2574,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省钱江人才科技计划(2007R10018)
