摘要
目的:观察电针经穴对脑缺血再灌注损伤大鼠不同时间点下丘脑-垂体-肾上腺(HPA)轴相关激素的影响,并初步探讨其作用机制。方法:将Wistar大鼠随机分为正常组、假手术组、模型组、电针非经穴组和电针经穴组,各组随机分成1 d、3 d、7 d 3个时间点,每时间点6只。采用改良线栓法制备大鼠大脑中动脉闭塞再灌注模型。电针经穴组取"曲池""足三里"穴,每次30 min,每日1次,取材前2 h再针刺1次。运用酶联免疫吸附实验方法检测大鼠血清皮质醇(CORT)含量变化,逆转录聚合酶链反应方法检测下丘脑促肾上腺皮质激素释放因子(CRF)、糖皮质激素受体(GR)及垂体促肾上腺皮质激素(ACTH)mRNA表达的差异。结果:与正常组比较,模型组各指标均有明显变化(P<0.05,P<0.01);与电针非经穴组和模型组各时间点比较,电针经穴组可明显降低CORT含量及CRF mRNA、ACTH mRNA表达水平(P<0.05,P<0.01),并可明显升高GR mRNA表达水平(P<0.01,P<0.05)。结论:电针经穴对脑缺血再灌注损伤大鼠HPA轴相关激素的调节具有相对特异性,电针经穴通过调控脑缺血再灌注损伤大鼠HPA轴功能紊乱起到脑保护作用。
Objective To observe the effect of electroacupuncture （EA） on the related hormones of the hypothalamus-pituitary-adrenal （HPA） axis in rats with cerebral ischemia-reperfusion injury （CI-RI）. Methods Ninety Wistar rats were randomly divided into normal control, sham-operation （sham）, model, EA acupoint and EA non-acupoint groups. CI-RI model was established by using modified middle cerebral artery occlusion （MCAO） and reperfusion. These rats were further divided into 1 day （d）, 3 d and 7 d subgroups, with 6 cases in each. EA （1 mA, 2 Hz/30 Hz） was applied to acupoint ＂Zusanli＂ （ST 36） and ＂Quchi＂ （LI 11）, and non-acupoints （5 mm lateral to ST 36 and 5 mm apart from LI 11 on the radial side respectively） for 30 min, once daily for 1 d, 3 d and 7 d separately. Serum cortisole （CORT） content was assayed by enzyme linked immunosorbent assay （ELISA）, and the expression of hypothalamic adrenocorticotropic releasing factor （CRF） mRNA and glucocorticoid receptor （GR） mRNA, and pituitary adrenocorticotrophic hormone （ACTH） mRNA was assayed by reverse transcription polymerase chain reaction （RT-PCR） method. Results Compared with normal and sham groups, serum CORT levels of model and EA non-acupoint groups on the 1^st, 3^rd and 7^Th day increased significantly （P〈0.05）, while compared with model groups, serum CORT level of EA acupoint groups decreased apparently （P〈0.05）. Compared with normal and sham groups, hypothalamic CRF mRNA and pituitary ACTH mRNA expression of model groups were upregulated significantly on the 1^st 3^rd and 7^th d after CI-RI （P〈0. 01, P〈0.05）, and hypothalamic GR mRNA expression of model groups were obviously downregulated （P〈0.01, P〈0.05）. In comparison with model groups, hypothalamic CRF mRNA and pituitary AOTH mRNA expression of EA acupoint groups at the 3 time-points, and their expression of EA non-acupoint groups on the 1^st and 3^rd day were remarkably downregulated （ P〈0.01, P〈0.05）, and hypothalamic GR mRNA expression of EA acupoint groups was upregulated significantly at the 3 time-points （P〈0.01, P〈0.05）. Comparison between EA acupoint and EA non-acupoint groups showed that the effect of EA acupoint groups was obviously superior to that of EA non-acupoint groups in downregulating ORF mRNA and AOTH mRNA expression and in upregulating GR mRNA expression at the 3 time-points （P〈0.01, P〈0.05）. Conclusion EA of meridian-acupoints can effectively downregulate serum OORT content, hypothalamic ORF mRNA and pituitary AOTH mRNA expression and upregulate hypothalamic GR mRNA expression in OI-RI rats, which may contribute to its effect in relieving OI-RI.
出处
《针刺研究》
CAS
CSCD
北大核心
2009年第5期297-303,共7页
Acupuncture Research
关键词
脑缺血再灌注
电针
经穴
下丘脑-垂体-肾上腺轴
激素
Cerebral ischemia reperfusion injury
Electroacupuncture
Meridian-acupoints
HPA axis
Serum cortisole
Hypothalamic CRF mRNA and GR mRNA
Pituitary AOTH mRNA
