摘要
目的:研究肝细胞生长因子(HGF)对转化生长因子β1(TGFβ-1)诱导肺泡上皮细胞转分化的影响。方法:应用TGF-β1诱导肺泡Ⅱ型上皮细胞转分化。将人肺腺癌A549细胞株培养液分为4组:①对照组;②HGF组;③TGFβ-1组;④HGF+TGFβ-1组,应用倒置相差显微镜观察细胞形态变化;免疫细胞化学方法检测肌成纤维细胞特异性标志物α-平滑肌肌动蛋白(α-SMA)、肺泡上皮细胞特异性标志物肺泡表面活性蛋白A(SP-A)的表达。结果:①TGF-β1组肺泡上皮细胞从原来典型的上皮细胞形态转变为长梭形肌成纤维细胞样形态。②TGF-β1组的细胞α-SMA的表达明显增加,SP-A的表达明显减低。③HGF组的细胞形态学、α-SMA、SP-A的表达与对照组无明显差异。④HGF+TGFβ-1组,多数细胞保持原来肺泡上皮细胞的形态,α-SMA表达明显低于TGFβ-1组、SP-A表达明显高于TGFβ-1组。结论:HGF能够在一定程度上负性调控TGFβ-1诱导的肺泡上皮细胞的转分化。
Objective： To observe the effect of hepatocyte growth factor（HGF） on transforming growth factor-β1（TGF-β1） triggered alveolar Ⅱcell transdifferentiation.Methods： Cultured cells from pulmonary adenocarcinoma cell line A549 were assigned into the following groups： control group,HGF group,TGF-β1 group,and HGF＋ TGF-β1 group.The morphology of transdifferentiated alveolar Ⅱ cells was observed using phase-contrast microscope and scanning electron microscope.SP-A and α-SMA were assessed by immunocytochemistry.Results： ①The morphology of cells in TGF-β1 group was altered from the appearances of cellula epithelialis to the appearances of myofibroblast.②The expression of α-SMA was increased but the expression of SP-A was decreased in TGF-β1 group,compared with that in control group respectively.③There was no statistically significant difference between HGF group and control group in the morphology of cells and the result of SP-A and α-SMA immunostaining.④The morphology of most cells in HGF＋ TGF-β...更多1 group still remained the appearances of cellula epithelialis,and the expression of α-SMA was decreased but the expression of SP-A was increased compared with that in TGF-β1 group respectively.Conclusion： HGF could block the alveolar Ⅱ cell transdifferentiation induced by TGF-β1,which implies that HGF might participate in the lung fibrosis as a negative regulator.
出处
《武汉大学学报（医学版）》
CAS
北大核心
2009年第5期566-569,F0002,共5页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:30670938)
