摘要
应用裂解气相色谱法和系统聚类分析对56株不同地方分离株昆虫病毒(其中NPV29株,CPV11株,GV16株)进行了识别分析.利用欧氏距离系数的8种系统聚类算法所得聚类树状图谱,结果表明,通过裂解指纹图特征峰的分析,可明显地区分NPV、CPV、GV彼此间的差异和相同亚群的不同分离株间的异同.用聚类分析进一步证明裂解气相色谱法对昆虫病毒识别的可行性,从而为病毒的分类鉴定提供了准确、快速、重复性好的一种现代分析方法。
The pyrolysis gas chromatography of 29 strains nuclear polyhedrosis viruses,11 strains cytoplasmic polyhedrosis viruses and 16 strains granulosis viruses were performed on a shimadzu GV-9A capillary gas chromatography equipped with a pyrolyser model PYR-2A. The differences among the inclusion bodies of NPV, CPV and GV could clearly be distinguished and also beidentified through analysis of fingerprinting the hierarchical clustering analysis of GC,This procedure has Provided a usefel method for the classification and identifcation of viruses.
出处
《微生物学报》
CAS
CSCD
北大核心
1998年第4期304-309,共6页
Acta Microbiologica Sinica
基金
中国科学院技条局资助
关键词
裂解气相色谱法
昆虫病毒
系统聚类分析
Pyrolysis gas chromatography, Insect virus, Hierarchical clustering analysis