摘要
链霉菌质粒pSGL1是本实验室在球孢链霉菌（Streptomycelglobisporus）中发现的新的质粒，它可以在变铅青链霉菌中复制。用缺失分析确定了pSGL1的基本复制区位于一个2．0kb的片段上。pSGL1能够与pIJ101相容。pSGL1是一个高拷贝质粒，拷贝数约为70～250。在对质粒进行分析的过程中得到的一些衍生质粒如pSGLN和pSGLS3等可以作为新的链霉菌基因克隆载体，并可用于构建新的高效分泌表达的链霉菌载体。
The plasmid pSGL1(7.4kb) which was isolated from antitumor antibiotic C-1027 producing strain Streptomyces globisporus C- 1027 was charaterized. It could be introduced into Streptomyces lividans by transformation. Following a series of deletion experiments,the minimal replication region was located within a 2.0kb San 3AI fragment. pSGL1 is compatible with pIJ01. pSGL1 and its derivatives have a copy number of 70～250. Some of the derived plasmids such as pSGLN and pSGLS3 have suitable selectable antibiotic resistance marker and unique restriction endonuclease sites and may be potentially useful as cloning vectors. These plasmids may be used to design and construct efficient secretory expression vectors.
出处
《微生物学报》
CAS
CSCD
北大核心
1998年第4期256-260,共5页
Acta Microbiologica Sinica
基金
中国医学科学院基金
关键词
链霉菌
pSGL1
复制区
相容性
拷贝数
Streptomyces pSGL1,Minimal replication region, Compatibility, Copynumber
