摘要
用１０种限制性内切酶对我国大陆５个地方（武汉、长沙、桂林、南宁和北海），６个黑腹果蝇（Ｄｒｏｓｏｐｈｉｌａｍｅｌａｎｏｇａｓｔｅｒ）群体的线粒体ＤＮＡ进行了限制片段长度多态性分析。在５６个单雌系中，发现了２５种不同的限制类型。应用Ｎｅｉ等（１９７９）的数学模型和ＵＰＧ法，构建了限制类型间和群体间的系统进化树。结果发现：所研究的群体分为３个类群，对应于南、中和北３个亚热带地区；除长沙玉合醋厂外，所有采自果品市场群体的群体内遗传距离（πｉ）表现出随纬度上升而增加的趋势。这些结果为探讨我国大陆黑腹果蝇的起源和演化提供了重要的依据。
The RLFPs of mtDNA from six Drosophila melanogaster populations in five places (Wuhan,Changsha,Guilin,Nanning and Beihai) were analysed with ten different restriction enzymes,25 restriction types were observed among 56 isofemale lines. By using Nei and Li’s mathematic model and UPG method,the phylogenetic trees for both the restriction types and populations were constructed based on their genetic distances. Both of the trees showed three cluster groups,corresponding to three climalic zones:the south,middle and north subtropical zone respectively. The genetic distance within population (π i ) except a vinegar plant population,increased with the geographic latitude. These results provid important information to study the origin and evolution of Chinese D. melanogaster.
基金
陕西自然科学基金
