摘要
目的研究MTT染色法用于检测与区分化疗后活的与凋亡的骨肉瘤细胞可行性。方法以手术切取的骨肉瘤组织的细胞建立凋亡细胞模型,采用MTT染色法检测,直接在光镜下进行形态学观察,并与荧光染色方法的观察结果进行比较。结果MTT染色法可准确判定化疗后活的与凋亡的骨肉瘤细胞,并可清晰分辨正常细胞、凋亡细胞和死亡细胞。结论MTT染色法是一种简单、可行的检测骨肉瘤细胞凋亡的方法,可用于化疗后肿瘤坏死率的研究。
Objective To assess the feasibility of using MTT assay for detecting the apoptosis ofosteosarcoma cells following chemotherapy. Methods The osteosarcoma cells derived form surgical specimens were cultured in RPMI 1640 medium supplemented with 10% calf serum. Chemotherapeutic agents were administered in the cell culture, and MTT assay was used to observe the cell apoptosis under optical microscope. Results MTT staining accurately identified apoptosis ofthe osteosareoma cells, and the apoptotic cells were easily distinguished from normal ceils and dead cells. Conclusion MTT staining is convenient and practical for detecting osteosarcoma cell apoptosis.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第9期1899-1901,共3页
Journal of Southern Medical University
基金
南方医院院长基金(20060009)
