摘要
目的采取球囊损伤加高胆固醇饮食方式建立兔腹主动脉粥样硬化模型并用多种方式评估,研究快速建模及无创评估模型的方法。方法16只雄性新西兰白兔分成实验组和对照组。球囊损伤腹主动脉后,实验组给予高胆固醇饲料喂养,对照组普食喂养,8周后应用病理切片、血管造影及体表超声等多种方式检测斑块成膜情况,分析其斑块性质。结果高胆固醇饮食8周后兔体重、总胆固醇、甘油三酯、低密度脂蛋白明显升高(P<0.001),腹主动脉造影及体表超声均能直接观察到实验组兔动脉粥样硬化斑块形成,实验组损伤部位与对照组损伤部位之间血管收缩率差异有显著性意义(t=5.921,P=0.000),体表超声测得偏心指数较大的斑块在药物刺激后血管收缩明显增加,偏心指数与血管收缩率之间呈显著正相关(r=0.983,P=0.000)。结论通过球囊损伤加高胆固醇饮食方式可以在短时间内建立兔动脉粥样硬化模型,且采取体表超声检测病变血管管壁厚度、管腔内径及血管偏心指数可以作为无创评价兔腹主动脉粥样硬化模型是否成模并初步筛选斑块性质的方式之一。
Objective To explore the methods for non-invasive evaluation of a rabbit model of aorta atherosclerosis model. Methods Sixteen male New Zealand rabbits （n=16） were randomized into the experimental group and control group and fed with high-cholesterol diet and normal diet after balloon injury in the abdominal aorta, respectively. Eight weeks later, pathological examination, angiography and surface ultrasonography were carried out to evaluate the plaques in the aorta. Results After feeding with high-cholesterol diet for 8 weeks, the weight, total cholesterol （TC）, triglycerides （TG） and low-density lipoprotein （LDL） were significantly increased in the rabbits （P〈0.001）, and atheroselerosis in the abdominal aorta was directly observed using angiography and surface ultrasonography. The rate of vasoconstriction showed significant difference between the experimental group and control group （t=5.921, P=-0.000）. In the experimental rabbits, the vasoconstriction increased obviously after drug stimulation with high lumen eccentricity index. A significant positive correlation was noted between the lumen eccentricity index and the rate of vasoconstriction （r=0.983, P=-0.000）. Conclusion A rabbit model of abdominal aorta atherosclerosis can be established rapidly by balloon injury and high-cholesterol diet. The aortic wall thickness, lumen diameter and lumen eccentricity index determined by surface ultrasonography can be used to evaluate the validity of the model establishment and the nature of the plaque.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第9期1874-1877,共4页
Journal of Southern Medical University
关键词
兔
动脉粥样硬化
疾病模型
动物
超声
血管造影
rabbit
atherosclerotic
disease models, animal
ultrasonography
angiography
