摘要
目的从细胞和分子水平观察隔药饼灸对兔高脂血症(HLP)合并动脉粥样硬化(AS)的作用机理。方法将75只兔随机分为5组,即空白组、模型组、直接灸组、隔药饼灸组、药物(血脂康)组,每组15只。采用免疫反应性损伤法,建立兔HLP合并AS模型。聚合酶链式反应(RT-PCR)测定主动脉血管细胞粘附因子E选择素(E-sel)的基因表达。结果隔药饼灸组、直接灸组及药物组均使兔HLP合并AS血管内皮细胞粘附因子E-selmRNA表达显著下调(P<0.05或P<0.01)。结论隔药饼灸对兔HLP合并AS的主动脉内皮细胞有一定的保护作用。
Objective To observe the mechanism of herbal cake separated moxibustion to hyperlipemia （HLP） and atherosclerosis （AS） rabbits on cell and molecular level. Method 75 rabbits were divided into 5 groups randomly： blank group, model group of HLP and AS, direct moxibustion group, herbal cake separated moxibustion group, herb group, 15 rabbits each group. Model rabbits of HLP and AS were built by immunity injury method. The expression of VCAM-1 mRNA and E-sel mRNA were determined with the method of RT-PCR. Results The expression of E-sel mRNA in herbal cake separated moxibustion group, direct moxibustion group and herb group are obviously decreased （P〈0.05 or P〈0.01）. Conclusion Herbal cake separated moxibustion has protective effect on the arotic endothelial cells of HLP and AS rabbits.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第7期37-39,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
湖南省教育厅重点资助项目(02A030)
关键词
隔药饼灸
高脂血症
动脉粥样硬化
E-选择素
herbal cake separated moxibustion
hyperlipemia
atherosclerosis
E-selectin
