摘要
目的研究多孔处理的新型镍钛合金材料与大鼠骨髓基质细胞(BMSCs)的生物相容性,探索新型生物工程材料的生物学特性。方法SD大鼠体外培养BMSCs,将细胞分别接种于致密、大孔、小孔组镍钛合金材料表面,建立体外共同培养。采用MTT法检测第3天BMSCs的增殖率;第7天采用Hoechst33342标记细胞,荧光显微镜下观察各材料组细胞数的差异;扫描电镜观察细胞在各组材料上的附着和生长情况。结果大孔、小孔组材料表面的细胞在第3天细胞增殖率和致密组相比有显著性差异(P<0.05),第7天在荧光纤维镜下发现各组镍钛合金材料上均有细胞生长,大孔、小孔组细胞数和致密组相比有显著性差异(P<0.05),大孔、小孔材料之间细胞增殖率和细胞数无统计学意义(P>0.05)。扫描电镜观察大孔、小孔组新型镍钛合金材料表面接种的BMSCs形态正常,增殖旺盛,黏附性良好。结论多孔处理的新型镍钛合金材料在体外实验中有良好的生物相容性,可促进BMSCs黏附、聚集和增殖。
Objective To investigate the biocompatibility of a novel cavernous nickel-titanium alloy with rat bone marrow stromal cells （BMSCs） in vitro. Methods Rat BMSCs were cultured on the surface of compact, microporous and macroporous nickel-titanium alloys, and the cell proliferation on day 3 during the culture was assessed using MTT assay. On day 7 of the cell culture, the cells were labeled with Hoechst33342 for cell counting under a fluorescence microscope. Scanning electron microscopy （SEM） was performed on day 7 of cell culture to observe the morphological changes of the cells. Results The cell proliferation rate and cell numbers differed significantly between the cavernous alloy groups and the compact alloy group （P〈0.05）, but similar between the former two groups （P〉0.05）. SEM showed that compared with the compact alloy, microporous and macroporous nickel-titanium alloys had better biocompatibility with the BMSCs, and the cells on the surface of the cavernous alloys had normal cell morphology. Conclusion Cavernous nickel-titanium alloy has good biocompatibility and can promote the adhesion, aggregation and proliferation of rat BMSCs in vitro.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2009年第9期1787-1789,共3页
Journal of Southern Medical University
基金
国家自然科学基金重点项目(U0632008)
关键词
镍钛合金
多孔
骨髓基质细胞
生物相容性
nickel-titanium alloy
cavernous
bone marrow stromal cells
biocompatibility
