摘要
目的探讨大鼠急性缺血性视网膜损伤中脑红蛋白（NGB）基因的表达及变化。方法随机将50-60日龄Wistar大鼠70只分为7组,左眼造模,制成高眼压诱导的视网膜急性缺血损伤模型,分别在眼压升高1、5、10、15、20、30、60 min迅速取视网膜,对侧眼作为自身正常对照直接取视网膜。提取视网膜组织总RNA,用半定量RT-PCR进行检测。结果检测中可见缺血1 min组,NGB mRNA表达较对照组迅速增加（P=0.017）,缺血5 min达高峰,与对照组比较差异有统计学意义（P=0.000）;10-15 min开始降低,但仍然保持在高水平（10 min,P=0.001;15 min,P=0.038）;20-30 min已迅速降低,但仍高于对照组（20 min,P=0.269;30 min,P=0.402）;60 min相对于对照组水平略有降低,与对照组比较差异无统计学意义（P=0.384）。结论大鼠视网膜急性缺血损伤后NGB mRNA表达增强,提示NGB可能在视网膜缺氧的适应性调节过程中起了重要作用。
Objective Neuroglobin is used to name the third kind of oxygen-carrying globulin,which mainly is expressed in brain, retina and endocrine tissue. Neuroglobin plays important roles during the hypoxic preconditioning, that is, the less neuroglobin level in tissue is,the lower the tolerable capacity of tissue to hypoxic condition. This study was designed to investigate the expression and change of neuroglobin （NGB） gene in hypoxic-isehemie retinal injury of rat. Methods Seventy Wistar rats were divided into 7 groups randomly. The experimental models of retinal hypoxic-ischemia injury were induced by elevating introcular pressure to 110 mmHg via anterior chamber eanula insertion to infuse the normal saline solution from the container in the height of 150 cm in the left eyes of 70 Wistar rats,and the fellow eyes worked as normal controls, The animals were sacrificed at 1 minute,5,10,15,20,30 and 60 minutes after retinal hypoxic-ischemia injury respectively and retinas were collected to extract the total RNA for the detect of NGB mRNA level using semiquantitative reverse transcription-PCR. The use of animal followed the Standard of Association for Research in Vision and Ophthalmology. Results The reverse transcription-PCR revealed that expression of neuroglobin mRNA in hypoxic-ischemic retina was consisted with the presenting value. A significant difference was found in expression of neuroglobin mRNA in hypoxic-ischemic retina among different time groups （F = 8. 036,P = 0. 000）. From 1 minute through 15 minutes after operation, the expression value of NGB mRNA was significantly higher than that of normal control group （P1min = 0. 017; P5min. = 0.000; P10min = 0.001; P15min = 0.038 ） with the peak expression in 5 minutes after operation. No remarkable difference was seen between 20,30,60 minutes group and normal control group （P20min = 0. 269 ;P30min = 0. 402;P60min= 0. 384）. Conclusion This study result suggests that NGB may play important function in the regulation of acute hypoxic-ischemic retinal injury.
出处
《眼科研究》
CSCD
北大核心
2009年第10期915-918,共4页
Chinese Ophthalmic Research
关键词
脑红蛋白
缺氧缺血
视网膜
neuroglobin
hypoxia-ischemia injury
retina
