摘要
背景与目的:研究香加皮杠柳苷(periplocin extracted from cortex periplocae,CPP)对人结肠癌SW480细胞株在裸鼠体内的成瘤抑制作用及其机制。材料与方法:将SW480细胞经皮下接种Balb/c-nu/nu裸小鼠,构建裸鼠SW480细胞荷瘤动物模型,观察CPP作用后移植瘤体积和重量的变化,HE染色光镜下观察移植瘤组织形态学变化,免疫组织化学方法检测瘤组织内Wnt/β-catenin信号通路中核心成分β-catenin及其下游靶蛋白Survivin和C-myc表达的变化。结果:CPP在体内能明显抑制SW480细胞荷瘤小鼠移植瘤的生长,其肿瘤体积和重量均被明显抑制,抑瘤率为61.41%(P<0.01)。CPP治疗组小鼠的移植瘤组织出现明显炎性细胞浸润和坏死性变化;肿瘤组织内β-catenin、Survivin和C-myc蛋白表达水平明显下降(P<0.05或P<0.01)。结论:CPP可抑制结肠癌细胞SW480在小鼠体内的增殖,其作用机制可能与Wnt/β-catenin信号分子的表达下调有关。
BACKGROUND AND AIM：This study was to explore the inhibitory effect of periplocin extracted from cortex periplocae（CPP） on tumor formation activity of human colorectal cancer cell line SW480 in nude mice,and to investigate its antitumor mechanisms.MATERIALS AND METHODS：SW480 cells were injected subcutaneously into nude mice to construct Balb/c-nu/nu mice model of SW480 cells.After treated with CPP,the transplanted tumor volume and weight of nude mice were measured.Morphology of SW480 cells in transplanted tumors were observed under light microscope after HE staining. Expression of β-catenin, Survivin and C-myc involved in Wnt/β-catenin signaling pathway were detected by immunohistochemistry. RESULTS： The growth of transplanted tumor in nude mice was inhibited markedly by CPP, the volume and weight of tumors were significantly less, the inhibition rate was 61.41% （P 〈 0.01）. There were inflamatory cells and noticeable necrosis in transplanted tumor tissue of CPP-treated mice. Expression levels of β-catenin, Survivin and C-myc in transplanted tumor were significantly lower in CPP-treated group than in control group. CONCLUSION： CPP exerted significant inhibitory effect on the proliferation of human colon cancer SW480 cells in nude mice. The mechanisms may be associated with down-regulating the Wnt/β-catenin signaling pathway.
出处
《癌变·畸变·突变》
CAS
CSCD
2009年第5期344-347,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助项目(30772752)
河北省自然科学基金资助项目(C2008000952)
