摘要
探讨应用搅拌式生物反应器培养技术体外扩增人胎盘间充质干细胞(hPDMCs)的方法。以hPDMCs为种子细胞,利用微载体悬浮法在搅拌式生物反应器内进行体外培养,同时设置静态培养的培养瓶为对照组,观察细胞的扩增速率、细胞代谢(乳酸、葡萄糖、培养液的pH值)的变化,检测生物反应器培养前、后干细胞的表面标记物。hPDMCs在搅拌式生物反应器内每代可以扩增(10.55±1.62)倍,明显高于对照组(6.10±0.11)倍的扩增值(P<0.05),且细胞生长代谢指标优于对照组;流式细胞仪检测应用搅拌式生物反应器,培养后细胞表面标记物的表达率无明显改变(P>0.05)。搅拌式生物反应器能够提供良好的细胞生长环境,建立用于hPDMCs的大量扩增的体外三维培养体系。
This study is aimed to expand human placenta-derived mesenchymal stem cells （hPDMCs） in a stirring bioreactor, hPDMCs were cultured on cyterdex-3 microcarrier in a stirring bioreactor, static culture flask for the control groups. The velocity of expansion and metabolism indexes （lactic acid, glucose, pH in medium） were detedcted. The surface marker of hPDMCs was detected by FACS. hPDMCs were expanded （10.55 ± 1.62） times cultivated in the stirring bioreactor per passage, markedly surpassed that of the control groups （6.10 ±0. 11 ） times （ P 〈 0. 05）, and there was no significant change of the surface markers of hPDMCs cultured in the stirring bioreactor （P 〉 0.05 ）. The stirring bioreactor could provide a better environment for hPDMCs expansion, showing great potential to be a 3D culture system for hPDMCs expansion in vitro.
出处
《中国生物医学工程学报》
CAS
CSCD
北大核心
2009年第5期749-753,共5页
Chinese Journal of Biomedical Engineering
基金
辽宁省自然科学基金(2006226031-211)
