摘要
目的:通过检测血清晚期氧化蛋白产物(AOPP)、丙二醛(MDA)、超氧化物歧化酶(SOD)和氧化低密度脂蛋白(ox-LDL)水平,探讨老年急性心肌梗死(AM I)发病与氧化应激的关系。方法:选择老年AM I 30例和不稳定心绞痛(UA)23例作为研究对象,老年健康自愿者36例作为对照。采用分光光度计法检测AOPP水平,硫代巴比妥酸法检测MDA含量,黄嘌岭氧化酶法测定SOD活力和ELISA双抗体夹心法检测ox-LDL水平。结果:老年AM I组和UA组的AOPP、MDA、SOD和ox-LDL水平均明显高于对照组(P<0.01或<0.05)。AM I组AOPP和ox-LDL水平均高于UA组(P<0.05),而MDA、SOD水平两组间无明显差异(P>0.05)。AM I组中冠状动脉单支病变亚组与多支病变亚组相比,各项指标均明显增高(P<0.05)。结论:老年AM I发生时具有明显的氧化应激反应,并与病情的严重程度有关。
Objective： To explore the relationship between acute myocardial infarction （AMI） and oxidative stress by detecting serum concentrations of advanced oxidation protein products （AOPP）, malonaldehyde （MDA）, superoxide dismutase （SOD）, and oxidized low density lipoprotein （ox -LDL） in the aged. Methods： The serum concentration of AOPP was measured by spectrophotometer, MDA by thiobarbituric acid method, SOD activity by xanthine oxidase method, and ox - LDL by double antibody sandwich ELISA in 30 aged patients with AMI, 27 aged patients with unstable angina （UA） , and 36 healthy controls （HC）. Results： Compared with HC group, serum levels of AOPP, MDA, SOD and ox -LDL in AMI group and UA group increased significantly （P 〈0.01 or P 〈 0.05）. There were significant differences in the serum concentrations of AOPP and ox - LDL between AMI group and UA group （P 〈 0.05）, while no difference in MDA content and SOD activity （P 〉 0.05）. In AMI group, all detected index of single - vessel - disease subgroup were higher than those of multiple - vessel - disease subgroup （P 〈 0.05）. Conclusion： An obvious oxidative stress reaction is happened in the onset of aged AMI, and is closely correlated with severity of disease. The change of AOPP and ox - LDL maybe has some diagnostic value for AMI in the aged.
出处
《西北国防医学杂志》
CAS
2009年第5期334-336,共3页
Medical Journal of National Defending Forces in Northwest China
基金
梅州市科技计划资助项目(2009B42)
关键词
急性心肌梗死
晚期氧化蛋白产物
氧化低密度脂蛋白
丙二醛
超氧化物歧化酶
Acute myocardial infarction
Advanced oxidation protein products
Oxidized low density lipoprotein
Malonaldehyde
Superoxide dismutase
