摘要
建立了同时测定猪肉中四环素族、氯霉素和克伦特罗的固相萃取-高效液相色谱（SPE-HPLC）测定方法，采用Oasis HLB固相萃取柱净化萃取，Diamonsil TM C18色谱柱分离，磷酸盐缓冲液（pH至2．5）-乙腈梯度洗脱，检测波长245nm。结果表明：该测定方法对土霉素、四环索、金霉素、氯霉素和克伦特罗测定的线性范围均为2～12μg／ml，基底加标回收率为93．6％～96．1％，检出限符合国标要求，分离度和重现性良好，可用于肉类食品安全与质量监控，方法简便、可靠、实用、覆盖面广。
A method determining the race tetracyclines, chloromycetin and clenbuterol in pork was developed. The method was based on SPE （Oasis HLB column）, separated by Diamonsil TM C18, and using a mobile phase of phosphate buffer solution-acetonitrile in gradient elution, detected with wavelength 245 nm. The results showed all the goal components presented linear relations in the scope of 2-12μg/ml. The matrix recoveries ranged from 93.6% to 96. 1%. The detection limits complied with National Standards. The established method is simple, reliable, practical and widely usable, and can be used to control meat product quality and security.
出处
《桂林电子科技大学学报》
2009年第5期406-409,共4页
Journal of Guilin University of Electronic Technology
基金
广西科学研究与技术开发计划项目(桂科攻0592007-4C)
