摘要
目的:研究缺氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)和Bcl-2/腺病毒E1B19kDa结合蛋白3(Bcl-2andadenovirus E1B19kDa interacting protein3,BNIP3)在非小细胞肺癌(non-small cell lung carcinoma,NSCLC)组织中的表达及其相关性。方法:收集45例新鲜NSCLC标本(含癌旁组织)和14例正常对照肺组织,采用半定量RT-PCR和SP免疫组织化学方法,测定HIF-1α和BNIP3的表达水平及分析二者相关性,并与组织类型、分化程度、淋巴结转移、TNM分期、年龄、吸烟等进行多因素分析。结果:肺癌肿瘤组织中HIF-1α、BNIP3的mRNA表达水平显著高于癌旁组织及正常对照肺组织(P<0.01),并且两者表达呈正相关(r=0.68,P<0.01);HIF-1α、BNIP3在肿瘤组织中的蛋白阳性表达率分别为53.3%、66.7%,显著高于癌旁组织及正常对照肺组织(P<0.01),且二者阳性率呈正相关(rs=0.38,P<0.01)。单因素分析发现HIF-1α与组织类型、TNM分期相关(P<0.05),而BNIP3在相关临床病理参数分组中无显著差异。结论:HIF-1α与BNIP3在NSCLC中高表达且呈正相关,推测二者的高表达在肺癌的发生发展中可能具有重要作用。
Objective：To investigate the expression of hypoxia inducible factor-1α （HIF-1α） and the Bcl-2/adenovirus E1B 19 kDa-interacting protein 3 （BNIP3） in human non-small cell lung carcinoma（NSCLC） and their correlation. Methods ：The expression of BNIP3 and HIF-1α in 45 tumor samples,45 para-cancer tissues and 14 control normal lung tissues were examined by reverse transcription-polymerase chain reaction analyses （RT-PCR） and SP immunohistochemical method. Muhivariable analysis was used to analyze the correlation of the HIF-1α and BNIP3 expression on histological types, differentiation,TNM clinical staging,lymphatic metastasis, age and cigarette smoking. Results：Compared with the control group,the mRNA levels of HIF-1α and BNIP3 in lung carcinoma tissues were much higher. There was a significant difference between tumors and the control （P 〈 0.01 ）. BNIP3 mRNA levels were positively correlated with HIF-1α levels（r=0.68,P 〈 0.01）. In lung carcinoma,the positive expression rates of HIF-1α and BNIP3 proteins were 53.3% and 66.7% respectively,which were higher than those in para-cancer tissues and normal lung tissues （P 〈 0.01）. The positive correlation between HIF-1α and BNIP3 expression was highly significant（rs=0.38 ,P 〈 0.01 ）. The expression of HIF-1α was correlated with histological types and TNM clinical staging （P 〈 0.05）. No significant correlation was found between BNIP3 expression and patients＇ clinicopathologic parameters. Conclusion：The high expression levels of HIF-1α and BNIP3 were closely related in non-small cell lung carcinomas, both of them played important roles in the carcinogenesis and development of lung carcinoma.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第11期1525-1529,1594,共6页
Journal of Nanjing Medical University(Natural Sciences)
