摘要
应用限制性内切酶ＢａｍＨＩ、ＥｃｏＲＩ和ＨｉｎｄⅢ消化鸡毒支原体ＤＮＡ，其电泳图谱能区别鸡毒支原体各株，比十二烷基磺酸钠—聚丙烯酰胺凝胶电泳（ＳＤＳ—ＰＡＧＥ）更敏感，表明限制性内切酶分析为鉴别鸡毒支原体毒株，进行分子流行病学调查及确定在商品代鸡中是否疫苗株代替了ＭＧ野毒株提供了非常有用的工具。
The electrophoretic patterns of the nucleic acids of five strains of Mycoplasm gallisepticum digested with the restriction enzymes BamHI,EcoRI and Hind Ⅲ were useful for differentiation of them The procedure was more sensitive than the sodium dodecyl sulfate—polyacrylamide gel electrophoresis(SDS—PAGE)technique The test indicated that the restriction endonuclease anylysis was a useful tool in making epidemical survey and might be useful in field studies to determine if the vaccine strain will replace wild type M gallisepticum in commercial layers
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
1998年第6期560-563,共4页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
鸡毒支原体
限制性内切酶
DNA
鸡
Mycoplasma gallisepticum, Restriction endonuclease analysis
